Genetics Department, Research School of Biological Sciences, Australian National University, Canberra City, Australian Capital Territory, 2601, Australia.
Appl Environ Microbiol. 1985 Apr;49(4):990-3. doi: 10.1128/aem.49.4.990-993.1985.
Rhizobium-Azospirillum interactions during establishment of Rhizobium-clover symbiosis were studied. When mixed cultures of Azospirillum and Rhizobium trifolii strains were simultaneously inoculated onto clover plants, no nodulation by R. trifolii was observed. R. trifolii ANU1030, which nodulated clover plants without attacking root hairs, i.e., does not cause root hair curling (Hac), did not show inhibition of nodulation when inoculated together with Azospirillum strains. Isolation of bacteria from surface-sterilized roots showed that azospirilla could be isolated both from within root segments and from nodules. Inhibition of nodulation could be mimicked by the addition of auxins to the plant growth medium.
研究了在建立根瘤菌-三叶草共生关系过程中根瘤菌-固氮螺菌的相互作用。当将固氮螺菌和三叶草根瘤菌菌株的混合培养物同时接种到三叶草植物上时，观察不到三叶草根瘤菌的结瘤。三叶草根瘤菌 ANU1030 能够在不攻击根毛的情况下结瘤三叶草植物，即不会引起根毛卷曲（Hac），当与固氮螺菌菌株一起接种时，不会抑制结瘤。从表面消毒的根部分离细菌表明，固氮螺菌既可以从根段内部也可以从根瘤中分离出来。在植物生长培养基中添加生长素可以模拟对结瘤的抑制。
