Center for Bioengineering and Pollution Control and Department of Civil Engineering, University of Notre Dame, Notre Dame, Indiana 46556-0767.
Appl Environ Microbiol. 1990 Sep;56(9):2684-91. doi: 10.1128/aem.56.9.2684-2691.1990.
The white rot fungus Phanerochaete chrysosporium produces extracellular ligninases as part of its idiophasic ligninolytic system. Agitation has been widely reported to suppress both ligninase production and lignin degradation. Results show that mechanical inactivation of ligninase is possibly the reason why ligninase accumulation is low or absent in agitated shake-flask cultures. Agitation seems to affect the catalytic activity of ligninase and has no apparent effect on either the rate of ligninase production or the physiology of P. chrysosporium. The detergents Tween 20, Tween 40, Tween 60, Tween 80, and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) are able to protect both purified ligninase and extant ligninase in culture fluids (free of biomass) against mechanical inactivation due to agitation. Addition of Tween 80 at the end of primary growth to agitated shake flasks containing either pelleted or immobilized mycelial cultures results in production and maintenance of high levels of ligninase activity over several days under conditions of high agitation. Possible mechanisms by which the detergents could protect ligninase are discussed.
白腐真菌糙皮侧耳产生细胞外木质素酶作为其专性木质素降解系统的一部分。搅拌已被广泛报道会抑制木质素酶的产生和木质素的降解。结果表明，木质素酶的机械失活可能是在搅拌摇瓶培养物中木质素酶积累低或不存在的原因。搅拌似乎会影响木质素酶的催化活性，对木质素酶的产生速率或糙皮侧耳的生理状态没有明显影响。表面活性剂吐温 20、吐温 40、吐温 60、吐温 80 和 3-[(3-胆酰胺丙基)二甲氨基]-1-丙磺酸钠（CHAPS）能够保护纯木质素酶和培养物（无生物质）中的现存木质素酶免受搅拌引起的机械失活。在含有颗粒状或固定化菌丝体培养物的搅拌摇瓶中，在初级生长结束时添加吐温 80，可在高搅拌条件下维持高水平的木质素酶活性数天。讨论了表面活性剂保护木质素酶的可能机制。
