浮游微生物和微污损生物群落在富营养化河口的活性测量。
Activity measurements of planktonic microbial and microfouling communities in a eutrophic estuary.
机构信息
Department of Marine Science, University of South Florida, St. Petersburg, Florida 33701.
出版信息
Appl Environ Microbiol. 1986 Jan;51(1):157-62. doi: 10.1128/aem.51.1.157-162.1986.
[H]thymidine incorporation, the rate of reduction of iodonitrotetrazolium violet (INT) to INT formazan normalized to DNA, and the ratio of ATP to DNA were adapted to measure the activity of attached and unattached microbial assemblages of Bayboro Harbor, Fla. Activity measurements by [H]thymidine incorporation were made of cells attached to polystyrene culture dishes, in unfiltered water samples, and in the <1-mum-filtered fraction. In most cases, the activity of attached cells was greater than that of unattached cells either in unfiltered water samples or in the <1-mum fraction. The calculated thymidine incorporation rates for cells in the >1-mum fraction were higher than those for cells either in unfiltered water or in the <1-mum-filtered fraction. By the rate of reduction of INT to INT formazan normalized to DNA and by ATP-to-DNA ratios, attached cells were also more active than cells in unfiltered water samples. These results indicate that the microenvironment afforded by attachment is a more beneficial habitat for microbial growth. Reasons for greater activity by natural populations of attached bacteria are discussed.
[H]胸苷掺入、碘硝基四唑紫（INT）还原为 INT 甲臜的速率与 DNA 归一化、以及 ATP 与 DNA 的比率，均适用于测量佛罗里达州 Bayboro Harbor 附着和非附着微生物组合的活性。通过 [H]胸苷掺入对附着在聚苯乙烯培养皿上的细胞、未过滤水样和 <1μm 过滤部分进行活性测量。在大多数情况下，附着细胞的活性无论是在未过滤水样中还是在 <1μm 部分中，均大于非附着细胞的活性。>1μm 部分中细胞的计算胸苷掺入率高于未过滤水或 <1μm 过滤部分中细胞的胸苷掺入率。通过 INT 还原为 INT 甲臜的速率与 DNA 归一化和 ATP 与 DNA 的比率，附着细胞也比未过滤水样中的细胞更活跃。这些结果表明，附着提供的微环境更有利于微生物生长。讨论了附着细菌自然种群活性更高的原因。
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