Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604.
Appl Environ Microbiol. 1986 Nov;52(5):982-6. doi: 10.1128/aem.52.5.982-986.1986.
Growth characteristics of a cellulolytic nitrogen-fixing bacterium isolated from a marine shipworm by Waterbury et al. (J. B. Waterbury, C. B. Calloway, and R. D. Turner, Science 221:1401-1403, 1983) are described. When grown microaerobically, the bacterium exhibited doubling times of about 2 days in cellulose-supplemented synthetic medium devoid of combined nitrogen. Maximum growth was reached 12 to 16 days after inoculation. Growth optima for pH, temperature, and NaCl concentration were 8.5, 30 to 35 degrees C, and 0.3 M, respectively. During growth the bacterium produced succinic acid (0.026%) and acetic acid (0.010%). Formic acid (0.010%) was produced during the stationary growth phase. No growth was observed when glucose was the sole carbon source. Cellobiose supported weak growth, while longer-chain-length cellodextrins supported extensive growth. Analysis of residual carbohydrates in the medium during growth indicated that the bacterium catabolized a terminal glucose moiety from the cellodextrin chain.
由 Waterbury 等人分离自海洋船蛆的一种纤维素固氮菌的生长特性。（J. B. Waterbury、C. B. Calloway 和 R. D. Turner，Science 221:1401-1403，1983）。当在微氧条件下生长时，该细菌在不含复合氮的纤维素补充合成培养基中的倍增时间约为 2 天。接种后 12 至 16 天达到最大生长。pH、温度和 NaCl 浓度的最佳生长条件分别为 8.5、30 至 35°C 和 0.3 M。在生长过程中，细菌产生琥珀酸（0.026%）和乙酸（0.010%）。在静止生长阶段产生甲酸（0.010%）。当葡萄糖是唯一的碳源时，没有观察到生长。纤维二糖支持微弱的生长，而更长链长的纤维素则支持广泛的生长。在生长过程中分析培养基中的残留碳水化合物表明，细菌从纤维素链上代谢末端的葡萄糖残基。
