Department of Plant Pathology, Oklahoma State University, Stillwater, Oklahoma 74078-0285.
Appl Environ Microbiol. 1987 Jan;53(1):183-4. doi: 10.1128/aem.53.1.183-184.1987.
A rapid filter paper dot-immunobinding assay was adapted to detect the wall-less mollicute Spiroplasma citri in medium, plants, or insects. Filter paper spotted with sample was incubated first in dilute antiserum, then in protein A-peroxidase, and finally in a substrate of 4-chloro-1-naphthol plus hydrogen peroxide. The detection limit averaged 2.3 x 10 CFU/ml in cultures, and S. citri was detected in single infected leafhoppers. This assay was less sensitive but more rapid and economical than an enzyme-linked immunosorbent assay.
一种快速滤纸片免疫斑点结合检测法被用来检测无细胞壁的软壁体螺旋体属柑橘支原体在培养基、植物或昆虫中的存在。滤纸片上的样本点首先用稀抗血清孵育，然后用蛋白 A-过氧化物酶孵育，最后用 4-氯-1-萘酚加过氧化氢作为底物。在培养物中的检测下限平均为 2.3 x 10 CFU/ml，并且在单个感染的叶蝉中检测到了 S. citri。与酶联免疫吸附测定法相比，该检测法的灵敏度较低，但速度更快，更经济。
