Beyer C F
J Immunol Methods. 1984 Feb 24;67(1):79-87. doi: 10.1016/0022-1759(84)90087-5.
A rapid and convenient procedure for the determination of the immunoglobulin class of mouse monoclonal antibodies is described. Hybridoma supernatants or other dilute solutions of monoclonal antibodies are spotted onto nitrocellulose membrane strips, and each strip is incubated with a different rabbit antiserum specific for a mouse Ig class. The strips are then incubated with protein A conjugated to peroxidase, and finally with the peroxidase substrate 4-chloro-1-naphthol. A positive reaction produces a dark blue spot. The procedure is sensitive, economical, fast, and safe. Many monoclonal antibodies can be tested at one time, and most of the reagents can be re-used. The procedure has been applied to a new series of monoclonal antibodies recognizing plasma membrane antigens of rat thymocytes.
本文描述了一种快速便捷的方法来测定小鼠单克隆抗体的免疫球蛋白类别。将杂交瘤细胞培养上清液或其他单克隆抗体稀释溶液点样到硝酸纤维素膜条上，每条膜条与针对小鼠免疫球蛋白类别的不同兔抗血清孵育。然后将膜条与过氧化物酶偶联的蛋白A孵育，最后与过氧化物酶底物4-氯-1-萘酚孵育。阳性反应会产生深蓝色斑点。该方法灵敏、经济、快速且安全。可同时检测多种单克隆抗体，并且大多数试剂可重复使用。该方法已应用于一系列识别大鼠胸腺细胞质膜抗原的新单克隆抗体。
