W. K. Kellogg Biological Station, Michigan State University, Hickory Corners, Michigan 49060.
Appl Environ Microbiol. 1987 Feb;53(2):371-4. doi: 10.1128/aem.53.2.371-374.1987.
Flowthrough reactor flasks are described that allow continuous low-level nutrient input to mixed anoxic sediments without dilution of the sediment. The flasks were tested by simulating sulfate inputs into sediments collected from a freshwater eutrophic lake. After an initial 2-day adaptation within the reactor system, rates of methane production and sulfate consumption were constant for the duration of a 12-day incubation. A sulfate input rate of 0.15 mmol liter of sediment day resulted in an equivalent rate of sulfate removal, which was unaffected by inputs of acetate (1.0 mmol liter of sediment day). The rate of methane production in control reactors, 0.18 mmol liter of sediment day, was doubled by the addition of acetate, whereas sulfate consumption was only stimulated by additions of high concentrations of sulfate plus acetate (1.5 and 1.0 mmol liter of sediment day, respectively). The reactor system appears to be effective in maintaining the balance between sulfate reduction and methane production in freshwater sediments and is potentially useful for study of the response of sediment populations to varying inputs of naturally occurring substrates, selected inhibitors, or xenobiotic compounds.
文中描述了一种流通式反应堆瓶，它可以在不稀释沉积物的情况下，向混合缺氧沉积物持续输入低浓度养分。通过模拟向取自富营养化淡水湖的沉积物中输入硫酸盐，对这些反应堆瓶进行了测试。在反应堆系统中适应了最初的 2 天后，甲烷生成和硫酸盐消耗的速率在 12 天的孵育过程中保持不变。硫酸盐输入速率为 0.15mmol 升沉积物天，导致硫酸盐的去除速率相同，而这不受乙酸盐的输入（1.0mmol 升沉积物天）的影响。在对照反应器中，甲烷的生成速率为 0.18mmol 升沉积物天，添加乙酸盐可使其增加一倍，而硫酸盐的消耗仅受高浓度硫酸盐和乙酸盐（分别为 1.5 和 1.0mmol 升沉积物天）添加的刺激。该反应堆系统似乎能有效地维持淡水沉积物中硫酸盐还原和甲烷生成之间的平衡，并且对于研究沉积物种群对天然存在的基质、选定抑制剂或外来化合物的不同输入的反应具有潜在的用途。
