用于同型发酵和异型发酵乳酸菌计数的鉴别培养基。
A differential medium for the enumeration of homofermentative and heterofermentative lactic Acid bacteria.
机构信息
Food Fermentation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, and Department of Food Science, North Carolina Agricultural Research Service, North Carolina State University, Raleigh, North Carolina 27695-7624.
出版信息
Appl Environ Microbiol. 1987 Jun;53(6):1382-4. doi: 10.1128/aem.53.6.1382-1384.1987.
Abstract
A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH(2)PO(4) (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A total of 21 Lactobacillus, Pediococcus, Leuconostoc, and Streptococcus species were correctly classified with the medium.
摘要
研制了一种用于鉴别同型发酵和异型发酵乳酸菌的培养基。培养基的基本成分为果糖（14mM）、KH2PO4（18mM）、溴甲酚绿（作为 pH 指示剂）和其他支持生长的营养物质。在琼脂培养基中，同型发酵菌的菌落呈蓝色到绿色，而异型发酵菌的菌落保持白色。该培养基可正确鉴定 21 种乳杆菌属、肠球菌属、明串珠菌属和链球菌属。
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