Adnan Mohd, Patel Mitesh, Hadi Sibte
Department of Clinical Nutrition, College of Applied Medical Sciences, University of Hail , Hail , Saudi Arabia.
Bapalal Vaidhya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University , Surat , Gujarat , India.
PeerJ. 2017 Mar 15;5:e3085. doi: 10.7717/peerj.3085. eCollection 2017.
Probiotic microorganisms are gaining global importance because of their use in the preparation of a nutraceutical or in the treatment of infections. As per the health industry demand, there is an urgent need for exploring new indigenous probiotic strains with its specific origin due to variation in gut microflora, different food habits and specific host-microbial interactions. The main objective of the present study was to isolate and identify a novel probiotic strain from the gut of fish and evaluate its potentiality as a potent probiotic.
The whole study was designed with the isolation of novel lactic acid bacterial strain from the gut of fish with their biochemical and molecular identifications. The potentiality of the isolated strain as a potent probiotic was carried out according to the parameters described in FAD/WHO guidelines for the evaluation of probiotics in food.
The isolated strain was confirmed as F2 on the basis of various biochemical and 16s rRNA gene sequencing methods. F2 was able to survive under highly acidic and bile salt concentration with the ability for the production of lipase and Bsh enzyme. It was also able to survive under simulated gastrointestinal conditions with the inhibition ability of various pathogens. The antioxidant potentiality with the cell surface hydrophobicity and cell aggregation ability confirms its potentiality as a potent probiotic. All the results detail the potency of F2 as a novel probiotic for a safer use.
The isolation of with probiotic potential from the gut of fish is a new approach and done for the first time. However, the whole study concluded that the isolated strain might be used as a novel probiotic in the food industry for the production of new probiotic products which imparts health benefits to the host.
由于益生菌微生物在营养保健品制备或感染治疗中的应用，其在全球范围内变得越来越重要。根据健康产业的需求，由于肠道微生物群的差异、不同的饮食习惯以及特定的宿主 - 微生物相互作用，迫切需要探索具有特定来源的新型本土益生菌菌株。本研究的主要目的是从鱼的肠道中分离和鉴定一种新型益生菌菌株，并评估其作为有效益生菌的潜力。
整个研究设计为从鱼的肠道中分离新型乳酸菌菌株，并进行生化和分子鉴定。根据食品中益生菌评估的粮农组织/世卫组织指南中描述的参数，对分离菌株作为有效益生菌的潜力进行评估。
通过各种生化和16s rRNA基因测序方法，确认分离菌株为F2。F2能够在高酸性和胆盐浓度下存活，具有产生脂肪酶和Bsh酶的能力。它还能够在模拟胃肠道条件下存活，并具有抑制各种病原体的能力。其抗氧化潜力以及细胞表面疏水性和细胞聚集能力证实了它作为有效益生菌的潜力。所有结果都详细说明了F2作为一种新型益生菌安全使用的潜力。
从鱼的肠道中分离具有益生菌潜力的菌株是一种新方法，并且是首次进行。然而，整个研究得出结论，分离出的菌株可在食品工业中用作新型益生菌，用于生产对宿主有益健康的新型益生菌产品。
