Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, Pennsylvania 19118.
Appl Environ Microbiol. 1987 Aug;53(8):1839-45. doi: 10.1128/aem.53.8.1839-1845.1987.
Pathogenic strains of Xanthomonas campestris pv. glycines which cause hypertrophy of leaf cells of susceptible soybean cultivars and nonpathogenic strains which do not cause hypertrophy were compared for their ability to produce indole compounds, including the plant hormone indole-3-acetic acid (IAA) in liquid media with or without supplementation with l-tryptophan. Several additional strains of plant-pathogenic xanthomonads and pseudomonads were also tested for IAA production to determine whether in vitro production of IAA is related to the ability to induce hypertrophic growth of host tissues. Indoles present in culture filtrates were identified by thin-layer chromatography, high-performance liquid chromatography, UV spectroscopy, mass spectroscopy, and gas chromatography-mass spectrometry and were quantitated by high-performance liquid chromatography. All strains examined produced IAA when liquid media were supplemented with l-tryptophan. The highest levels of IAA were found in culture filtrates from the common bean pathogen Pseudomonas syringae pv. syringae, and this was the only bacterium tested which produced IAA without addition of tryptophan to the medium. Additional indoles identified in culture filtrates of the various strains included indole-3-lactic acid, indole-3-aldehyde, indole-3-acetamide, and N-acetyltryptophan. Pseudomonads and xanthomonads could be distinguished by the presence of N-acetyltryptophan, which was found only in xanthomonad culture filtrates.
比较了引起敏感大豆品种叶片细胞肥大的野油菜黄单胞菌 pv. 大豆致病变种和不引起肥大的非致病变种在有或没有补充 l-色氨酸的液体培养基中产生吲哚化合物的能力，包括植物激素吲哚-3-乙酸（IAA）。还测试了几种其他植物病原黄单胞菌和假单胞菌菌株的 IAA 产生情况，以确定体外产生 IAA 是否与诱导宿主组织肥大生长的能力有关。通过薄层层析、高效液相色谱、紫外光谱、质谱和气相色谱-质谱鉴定培养滤液中的吲哚，并通过高效液相色谱定量。当液体培养基中补充 l-色氨酸时，所有检查的菌株都产生 IAA。在普通菜豆病原体丁香假单胞菌 pv. 丁香假单胞菌的培养滤液中发现了最高水平的 IAA，这是唯一一种在不向培养基中添加色氨酸的情况下产生 IAA 的细菌。在各种菌株的培养滤液中还鉴定出其他吲哚，包括吲哚-3-乳酸、吲哚-3-醛、吲哚-3-乙酰胺和 N-乙酰色氨酸。假单胞菌和黄单胞菌可以通过存在 N-乙酰色氨酸来区分，这种物质只存在于黄单胞菌的培养滤液中。
