Unité de Mycologie and Station Centrale de Microscopie Electronique, Institut Pasteur, 75724 Paris Cedex 15, France, and Institut für Biotechnologie, Eidgenosische Technische Holzschule-Hönggerberg, CH-8093 Zurich, Switzerland.
Appl Environ Microbiol. 1987 Oct;53(10):2384-7. doi: 10.1128/aem.53.10.2384-2387.1987.
Using an anti-lignin peroxidase antiserum-protein A-gold complex, we found lignin peroxidase mainly intracellularly in several white rot fungi colonizing sawdust under laboratory conditions. This enzyme was also present in fungi found in naturally decayed wood. However, in all cases, lignin peroxidase was located mainly inside the fungal cells. Labeled lignin peroxidase did not bind to the lignocellulosic samples tested, with the exception of poplar milled-wood lignin. These results are discussed in relation to the role of lignin peroxidase during wood degradation.
利用抗木质素过氧化物酶抗血清-蛋白 A-金复合物，我们发现几种白腐真菌在实验室条件下分解木屑时，木质素过氧化物酶主要存在于细胞内。这种酶也存在于天然腐朽木材中的真菌中。然而，在所有情况下，木质素过氧化物酶主要位于真菌细胞内部。标记的木质素过氧化物酶不会与所测试的木质纤维素样品结合，除了杨木磨木木质素。这些结果与木质素过氧化物酶在木材降解过程中的作用有关。
