Barclay C D, Farquhar G F, Legge R L
Department of Chemical Engineering, University of Waterloo, Ontario, Canada.
Appl Microbiol Biotechnol. 1995 Mar;42(6):958-63. doi: 10.1007/BF00191197.
The ability of the white-rot fungus Phanerochaete chrysosporium (INA-12) to degrade various polynuclear aromatic hydrocarbons (PAH) was investigated. Under static, non-nitrogen-limiting conditions, P. chrysosporium mineralized both phenanthrene and benzo[a]pyrene. Total mineralization, based on radioactive tracing, was limited to 1.8%-3% for phenanthrene and benzo[a]pyrene respectively. In both cases the pattern of mineralization did not correlate temporally with the production of lignin peroxidase activity. Sorption of radiolabelled material to the biomass was very significant with 22% and 40% of the total radioactivity being sorbed for benzo[a]pyrene and phenanthrene respectively. A number of models were examined to predict the sorption isotherms, the best performance being obtained with a three-parameter empirical model. It is apparent that lignin peroxidase is not necessarily involved in the biodegradation of all PAH and that a significant factor in PAH biodegradation and/or disappearance in cultures with the intact fungus may be attributed to sorption phenomena.
研究了白腐真菌黄孢原毛平革菌（INA - 12）降解各种多环芳烃（PAH）的能力。在静态、非氮限制条件下，黄孢原毛平革菌使菲和苯并[a]芘均发生矿化。基于放射性示踪，菲和苯并[a]芘的总矿化率分别限制在1.8% - 3%。在这两种情况下，矿化模式在时间上与木质素过氧化物酶活性的产生均不相关。放射性标记物质在生物量上的吸附非常显著，苯并[a]芘和菲分别有22%和40%的总放射性被吸附。研究了多个模型来预测吸附等温线，用一个三参数经验模型获得了最佳性能。显然，木质素过氧化物酶不一定参与所有PAH的生物降解，并且在含有完整真菌的培养物中，PAH生物降解和/或消失的一个重要因素可能归因于吸附现象。
