Nicole M, Chamberland H, Geiger J P, Lecours N, Valero J, Rio B, Ouellette G B
Forestry Canada, Quebec Region, Sainte-Foy.
Appl Environ Microbiol. 1992 May;58(5):1727-39. doi: 10.1128/aem.58.5.1727-1739.1992.
The cellular distribution of laccase L1 during degradation of wood chips by Rigidoporus lignosus, a tropical white rot fungus, was investigated by using anti-laccase L1 polyclonal antisera in conjunction with immunolabeling techniques. The enzyme was localized in the fungal cytoplasm and was associated with the plasmalemma and the fungal cell wall. An extracellular sheath, often observed around fungal cells, often contained laccase molecules. Diffusion of laccase within apparently unaltered wood was seldom observed. The enzyme penetrated all degraded cell walls, from the secondary wall toward the primary wall, including the middle lamella. Xylem cells showing advanced stages of decay were sometimes devoid of significant labeling. These data suggest that the initial attack on wood was not performed by laccase L1 of R. lignosus. Previous alteration of the lignocellulose complex may facilitate the movement of laccase within the wood cell walls. This immunogold study revealed that laccase localization during wood degradation seems limited not in space but in time.
利用抗漆酶L1多克隆抗血清结合免疫标记技术，研究了热带白腐真菌木蹄层孔菌在降解木片过程中漆酶L1的细胞分布。该酶定位于真菌细胞质中，并与质膜和真菌细胞壁相关。在真菌细胞周围经常观察到的细胞外鞘中通常含有漆酶分子。在明显未改变的木材中很少观察到漆酶的扩散。该酶从次生壁向初生壁，包括胞间层，穿透所有降解的细胞壁。显示出高级腐烂阶段的木质部细胞有时没有明显的标记。这些数据表明，木蹄层孔菌的漆酶L1并非对木材进行初始攻击的酶。木质纤维素复合体先前的改变可能有助于漆酶在木材细胞壁内的移动。这项免疫金研究表明，木材降解过程中漆酶的定位似乎不是受空间限制，而是受时间限制。
