Department of Food Science, Cornell University, Ithaca, New York 14853.
Appl Environ Microbiol. 1988 Jun;54(6):1610-1. doi: 10.1128/aem.54.6.1610-1611.1988.
An argB mutant of Aspergillus oryzae NRRL 492 has been genetically transformed with the Aspergillus nidulans argB gene. Protoplasts were generated with a combination of Novozyme 234 and beta-glucuronidase and regenerated on sucrose-stabilized minimal medium without arginine as described for A. nidulans. A frequency of 5 to 10 transformants per mug of DNA was obtained; however, most transformants appeared abortive. The A. nidulans argB gene and vector sequences appeared to be integrated into the A. oryzae chromosome.
米曲霉 NRRL492 的 argB 突变体已通过天蓝色链霉菌 argB 基因进行遗传转化。正如天蓝色链霉菌所述，先用 Novozyme 234 和β-葡聚糖酶生成原生质体，然后在不含精氨酸的蔗糖稳定的最低培养基上再生。获得了每毫克 DNA 5 到 10 个转化体的频率；然而，大多数转化体似乎是无效的。天蓝色链霉菌 argB 基因和载体序列似乎已整合到米曲霉的染色体中。
