Ward M, Wilkinson B, Turner G
Mol Gen Genet. 1986 Feb;202(2):265-70. doi: 10.1007/BF00331648.
An allele (oliC31) of the A. nidulans oliC gene has been cloned using homology with the equivalent gene from N. crassa. OliC31 codes for an oligomycin-resistant, triethyltin-hypersensitive form of subunit 9 of the mitochondrial ATP synthase complex. Direct selection for oligomycin-resistance was possible following transformation of A. nidulans with the oliC31 gene. The phenotypes of transformants cultured in the presence of oligomycin were indicative of the position of integration of the transforming plasmid within the genome. Subsequent recombination events involving the integrated oliC31 gene were also apparent from altered levels of resistance to oligomycin or triethyltin. This gene should prove useful as a marker for transformation of strains lacking auxotrophic lesions and in gene replacement or disruption experiments.
利用与粗糙脉孢菌（Neurospora crassa）中同源基因的同源性，已克隆出构巢曲霉（Aspergillus nidulans）oliC基因的一个等位基因（oliC31）。OliC31编码线粒体ATP合酶复合体亚基9的一种对寡霉素有抗性、对三乙锡超敏感的形式。用oliC31基因转化构巢曲霉后，可以直接筛选对寡霉素有抗性的菌株。在寡霉素存在的情况下培养的转化体的表型表明了转化质粒在基因组中的整合位置。涉及整合的oliC31基因的后续重组事件也可从对寡霉素或三乙锡抗性水平的改变中明显看出。该基因应被证明可作为缺乏营养缺陷型损伤菌株转化的标记，以及用于基因替换或破坏实验。
