Savannah River Laboratory, E. I. du Pont de Nemours & Co., Inc., Aiken, South Carolina 29808, and Institute for Applied Microbiology, University of Tennessee, Knoxville/Oak Ridge National Laboratory, Knoxville, Tennessee 37932.
Appl Environ Microbiol. 1988 Jul;54(7):1709-14. doi: 10.1128/aem.54.7.1709-1714.1988.
Microbial consortia capable of aerobically degrading more than 99% of exogenous trichloroethylene (TCE) (50 mg/liter) were collected from TCE-contaminated subsurface sediments and grown in enrichment cultures. TCE at concentrations greater than 300 mg/liter was not degraded, nor was TCE used by the consortia as a sole energy source. Energy sources which permitted growth included tryptone-yeast extract, methanol, methane, and propane. The optimum temperature range for growth and subsequent TCE consumption was 22 to 37 degrees C, and the pH optimum was 7.0 to 8.1. Utilization of TCE occurred only after apparent microbial growth had ceased. The major end products recovered were hydrochloric acid and carbon dioxide. Minor products included dichloroethylene, vinylidine chloride, and, possibly, chloroform.
从受三氯乙烯（TCE）污染的地下沉积物中收集了能够好氧降解超过 99%的外源三氯乙烯（TCE）（50mg/L）的微生物群落，并在富集培养物中进行培养。浓度大于 300mg/L 的 TCE 不会被降解，群落也不会将 TCE 用作唯一能源。允许生长的能源包括胰蛋白胨-酵母提取物、甲醇、甲烷和丙烷。生长和随后 TCE 消耗的最佳温度范围为 22 至 37°C，最佳 pH 值为 7.0 至 8.1。只有在明显的微生物生长停止后，才会利用 TCE。回收的主要终产物是盐酸和二氧化碳。次要产物包括二氯乙烯、偏二氯乙烯和可能的氯仿。
