厌氧代谢气态终产物放射性同位素分析的快速方法。
Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism.
作者信息
Nelson D R, Zeikus J G
出版信息
Appl Microbiol. 1974 Aug;28(2):258-61. doi: 10.1128/am.28.2.258-261.1974.
Abstract
A gas chromatographic procedure for the simultaneous analysis of (14)C-labeled and unlabeled metabolic gases from microbial methanogenic systems is described. H(2), CH(4), and CO(2) were separated within 2.5 min on a Carbosieve B column and were detected by thermal conductivity. Detector effluents were channeled into a gas proportional counter for measurement of radioactivity. This method was more rapid, sensitive, and convenient than gas chromatography-liquid scintillation techniques. The gas chromatography-gas proportional counting procedure was used to characterize the microbial decomposition of organic matter in anaerobic lake sediments and to monitor (14)CH(4) formation from H(2) and (14)CO(2) by Methanosarcina barkeri.
摘要
描述了一种用于同时分析来自微生物产甲烷系统的含¹⁴C标记和未标记代谢气体的气相色谱方法。氢气、甲烷和二氧化碳在Carbosieve B柱上2.5分钟内实现分离，并通过热导检测。检测器流出物导入气体正比计数器以测量放射性。该方法比气相色谱 - 液体闪烁技术更快速、灵敏且方便。气相色谱 - 气体正比计数法用于表征厌氧湖泊沉积物中有机物的微生物分解，并监测巴氏甲烷八叠球菌由氢气和¹⁴CO₂生成¹⁴CH₄的过程。
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