两种嗜热乙酸营养型产甲烷菌（CALS-1 热甲烷八叠球菌和 CALS-1 热甲基八叠球菌）利用乙酸的动力学。
Kinetics of Acetate Utilization by Two Thermophilic Acetotrophic Methanogens: Methanosarcina sp. Strain CALS-1 and Methanothrix sp. Strain CALS-1.
机构信息
Department of Microbiology, Stocking Hall, Cornell University, Ithaca, New York 14853-7201.
出版信息
Appl Environ Microbiol. 1989 Feb;55(2):488-91. doi: 10.1128/aem.55.2.488-491.1989.
The kinetics of acetate utilization were examined for washed concentrated cell suspensions of two thermophilic acetotrophic methanogens isolated from a 58 degrees C anaerobic digestor. Progress curves for acetate utilization by cells of Methanosarcina sp. strain CALS-1 showed that the utilization rate was concentration independent (zero order) above concentrations near 3 mM and that acetate utilization ceased when a threshold concentration near 1 mM was reached. Acetate utilization by cells of Methanothrix sp. strain CALS-1 was concentration independent down to 0.1 to 0.2 mM, and threshold values of 12 to 21 muM were observed. Typical utilization rates in the concentration-independent stage were 210 and 130 nmol min mg of protein for the methanosarcina and the methanothrix, respectively. These results are in agreement with a general model in which high acetate concentrations favor Methanosarcina spp., while low concentrations favor Methanothrix spp. However, acetate utilization by these two strains did not follow simple Michaelis-Menton kinetics.
我们考察了从 58°C 厌氧消化器中分离得到的两种嗜热乙酸营养型产甲烷菌的浓缩洗涤细胞悬浮液中乙酸利用的动力学。Methanosarcina sp. 菌株 CALS-1 细胞利用乙酸的进展曲线表明，在接近 3mM 的浓度下，利用速率与浓度无关（零级），当达到接近 1mM 的阈值浓度时，乙酸利用停止。Methanothrix sp. 菌株 CALS-1 的乙酸利用在低至 0.1 至 0.2mM 的浓度下与浓度无关，观察到 12 至 21μM 的阈值。在无浓度依赖性阶段，产甲烷菌和产甲烷菌的典型利用速率分别为 210 和 130nmol min mg 蛋白。这些结果与一个普遍的模型一致，即高浓度的乙酸有利于 Methanosarcina spp.，而低浓度的乙酸有利于 Methanothrix spp.。然而，这两种菌株的乙酸利用并不遵循简单的米氏动力学。
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