Kamagata Y, Kawasaki H, Oyaizu H, Nakamura K, Mikami E, Endo G, Koga Y, Yamasato K
Fermentation Research Institute, Agency of Industrial Science and Technology, Ibaraki, Japan.
Int J Syst Bacteriol. 1992 Jul;42(3):463-8. doi: 10.1099/00207713-42-3-463.
Three thermophilic Methanothrix ("Methanosaeta") strains, strains PTT (= DSM 6194T) (T = type strain), CALS-1 (= DSM 3870), and Z-517 (= DSM 4774), were characterized chemotaxonomically and compared with five mesophilic strains, Methanothrix soehngenii ("Methanosaeta concilii") GP6 (= DSM 3671), Opfikon (= DSM 2139), FE (= DSM 3013), UA, and PM. These methanogens were exclusively acetotrophic and had a characteristic sheathed structure. The DNA base compositions of the strains which we studied ranged from 50.3 to 54.3 mol% guanine plus cytosine. The thermophilic strains often had phase-refractive gas vesicles inside their cells. Denaturing electrophoresis of proteins showed that the mesophilic and thermophilic Methanothrix strains formed two distinct groups and that there were differences in protein patterns between the groups. The difference between the thermophiles and mesophiles was also verified by comparing partial 16S rRNA sequences (ca. 30 base differences in ca. 540 bases). On the basis of our results, we propose the name Methanothrix thermophila for the three thermophilic strains. The type strain of M. thermophila is strain PT (= DSM 6194). We also propose that the name Methanothrix thermoacetophila ("Methanosaeta thermoacetophila"), which was given to strain Z-517 (type strain), should be rejected because of its description, which was based on an enrichment culture, was inadequate.
对嗜热甲烷丝状菌（“甲烷鬃菌属”）的3个菌株，即PTT（=DSM 6194T）（T=模式菌株）、CALS-1（=DSM 3870）和Z-517（=DSM 4774）进行了化学分类学特征分析，并与5个嗜温菌株，即索氏甲烷丝状菌（“和解甲烷鬃菌”）GP6（=DSM 3671）、奥菲孔（=DSM 2139）、FE（=DSM 3013）、UA和PM进行了比较。这些产甲烷菌均为专性乙酸营养型，且具有特征性的鞘状结构。我们研究的菌株的DNA碱基组成范围为鸟嘌呤加胞嘧啶含量50.3至54.3摩尔%。嗜热菌株的细胞内常有折光性的气体小泡。蛋白质变性电泳显示，嗜温与嗜热甲烷丝状菌菌株形成两个不同的组，且两组之间的蛋白质图谱存在差异。通过比较部分16S rRNA序列（约540个碱基中约有30个碱基差异）也证实了嗜热菌与嗜温菌之间的差异。根据我们的结果，我们提议将这3个嗜热菌株命名为嗜热甲烷丝状菌。嗜热甲烷丝状菌的模式菌株为PT（=DSM 6194）菌株。我们还提议废弃“嗜热乙酸甲烷丝状菌”（“嗜热乙酸甲烷鬃菌”）这一名称，该名称曾用于Z-517（模式菌株），因其描述基于富集培养，不够充分。
