Culture Collection and Research Center, Food Industry Research and Development Institute, P.O. Box 246, Hsinchu, Taiwan, Republic of China.
Appl Environ Microbiol. 1989 Dec;55(12):3167-72. doi: 10.1128/aem.55.12.3167-3172.1989.
An alpha-amylase gene (AMY) was cloned from Schwanniomyces occidentalis CCRC 21164 into Saccharomyces cerevisiae AH22 by inserting Sau3AI-generated DNA fragments into the BamHI site of YEp16. The 5-kilobase insert was shown to direct the synthesis of alpha-amylase. After subclones containing various lengths of restricted fragments were screened, a 3.4-kilobase fragment of the donor strain DNA was found to be sufficient for alpha-amylase synthesis. The concentration of alpha-amylase in culture broth produced by the S. cerevisiae transformants was about 1.5 times higher than that of the gene donor strain. The secreted alpha-amylase was shown to be indistinguishable from that of Schwanniomyces occidentalis on the basis of molecular weight and enzyme properties.
从施氏假丝酵母 CCRC 21164 中克隆了α-淀粉酶基因（AMY），并通过将 Sau3AI 生成的 DNA 片段插入 YEp16 的 BamHI 位点，将其导入酿酒酵母 AH22。该 5kb 插入片段可指导α-淀粉酶的合成。在筛选出含有各种长度受限片段的亚克隆后，发现供体菌株 DNA 的 3.4kb 片段足以进行α-淀粉酶的合成。与基因供体菌株相比，酿酒酵母转化体产生的发酵液中α-淀粉酶的浓度约高 1.5 倍。根据分子量和酶特性，分泌的α-淀粉酶与施氏假丝酵母的α-淀粉酶无法区分。
