IMC/Pitman Moore Incorporated, Terre Haute, Indiana 47808.
Appl Environ Microbiol. 1990 Mar;56(3):623-7. doi: 10.1128/aem.56.3.623-627.1990.
A mutant derivative of a novel pseudomonad isolated from the soil accumulated acetophenone when supplied with cinnamic acid. The microorganism has been identified as an unclassified Pseudomonas sp., similar to Pseudomonas acidovorans. Mass spectrum analysis of the product acetophenone derived from catabolism of cinnamic acid in the presence of O(2) or H(2)O supported the conclusion that cinnamic acid degradation is initiated by addition of water to the double bond of the side chain, followed by dehydrogenation to generate 3-keto-3-phenylpropionic acid. The intermediate 3-keto-3-phenylpropionic acid is accumulated in cultures of the mutant during active cinnamic acid catabolism. However, this intermediate is unstable so a portion of it spontaneously decarboxylates to form acetophenone. Neither 3-keto-3-phenylpropionic acid nor acetophenone is a precedented intermediate in cinnamic acid degradation. Isolation of the novel strain and mutant provide the rudiments for a process to produce natural acetophenone by biotransformation of natural cinnamic acid.
从土壤中分离到的新型假单胞菌的突变体衍生物在提供肉桂酸时会积累苯乙酮。该微生物已被鉴定为未分类的假单胞菌属，类似于产酸假单胞菌。在 O(2)或 H(2)O 存在下，对来自肉桂酸分解的产物苯乙酮的质谱分析支持了这样的结论，即肉桂酸的降解是通过在侧链的双键上加水分子引发的，然后脱氢生成 3-酮-3-苯基丙酸。在突变体的肉桂酸代谢活跃的培养物中积累了中间产物 3-酮-3-苯基丙酸。然而，该中间产物不稳定，因此一部分会自发脱羧形成苯乙酮。3-酮-3-苯基丙酸和苯乙酮都不是肉桂酸降解中的预期中间产物。新型菌株和突变体的分离为通过天然肉桂酸的生物转化生产天然苯乙酮提供了基础。
