Department of Plant Pathology, University of Arizona, Tucson, Arizona 85721, and Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108.
Appl Environ Microbiol. 1990 Jun;56(6):1932-43. doi: 10.1128/aem.56.6.1932-1943.1990.
Isolates of six species of Ganoderma in the G. lucidum complex were evaluated for their ability to decay wood of Quercus hypoleucoides A. Camus and Abies concolor (Gord. and Glend.) Lindl. ex. Hildebr. by using in vitro agar block decay tests. Morphological, ultrastructural, and chemical studies of decayed wood were used to determine the extent of delignification or simultaneous decay caused by each species of Ganoderma. All species decayed both white fir and oak wood; however, less percent weight loss (%WL) occurred in white fir than oak. In white fir, isolates of two undescribed Ganoderma species (RLG16161, RLG16162, JEA615, and JEA625) caused significantly higher%WL (21 to 26%) than that in G. colossum, G. oregonense, G. meredithiae, and G. zonatum (10 to 16%). Only Ganoderma sp. isolates JEA615 and JEA625 caused delignification, with JEA615 causing a lignin-to-glucose gram loss ratio of 1.6:1. Morphological and ultrastructural studies confirmed delignification by this fungus and showed that some delignification had occurred by all of the species, although areas of delignification were limited to small regions adjacent to simultaneously decayed cells. In oak, G. colossum caused significantly less%WL (22 to 35%) than the other species (38 to 52%). All of the species, except G. meredithiae, caused delignification with lignin-to-glucose gram loss ratios ranging from 1.4 to 4.9:1. Extensive delignification by isolates of G. colossum and G. oregonense was observed; moderate delignification was caused by the other species. Ganoderma meredithiae caused a simultaneous decay, with only small localized regions of cells delignified, while delignification by G. zonatum was irregular, with specific zones within the cell wall delignified. The thermophilic and chlamydosporic G. colossum has the capacity to cause extensive delignification and appears ideally suited for use in lignin degradation studies and biotechnological applications of lignin-degrading fungi.
六种灵芝复合体中灵芝物种的分离物被评估其分解白枞和栎木的能力，使用体外琼脂块分解测试。对分解木材的形态学、超微结构和化学研究用于确定每个灵芝物种引起木质素脱除或同时分解的程度。所有物种都分解了白枞和栎木，但白枞的重量损失百分比（%WL）比栎木低。在白枞中，两种未描述的灵芝物种（RLG16161、RLG16162、JEA615 和 JEA625）的分离物引起的%WL（21%至 26%）显著高于 G. colossum、G. oregonense、G. meredithiae 和 G. zonatum（10%至 16%）。只有 Ganoderma sp.分离物 JEA615 和 JEA625 引起木质素脱除，其中 JEA615 引起木质素到葡萄糖克损失比为 1.6:1。形态学和超微结构研究证实了该真菌的木质素脱除，并表明所有物种都发生了一些木质素脱除，尽管木质素脱除区域仅限于与同时分解细胞相邻的小区域。在栎木中，G. colossum 引起的%WL（22%至 35%）显著低于其他物种（38%至 52%）。除 G. meredithiae 外，所有物种都引起木质素脱除，木质素到葡萄糖克损失比范围为 1.4 至 4.9:1。G. colossum 和 G. oregonense 的分离物引起广泛的木质素脱除，而其他物种引起中等程度的木质素脱除。G. meredithiae 引起同时分解，只有小的局部区域的细胞木质素脱除，而 G. zonatum 的木质素脱除不规则，细胞壁内的特定区域木质素脱除。嗜热和厚壁孢子的 G. colossum 具有引起广泛木质素脱除的能力，似乎非常适合用于木质素降解研究和木质素降解真菌的生物技术应用。
