Ibrahim Darah, Zhu Han Li, Yusof Nuraqilah, Hong Lim Sheh
Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Faculty of Pharmacy, Universitas Air Langga, Surabaya, Indonesia.
Trop Life Sci Res. 2013 Aug;24(1):71-84.
A total of 34 bacterial isolates were obtained from soil samples collected from Changar Hot Spring, Malang, Indonesia. Of these, 13 isolates produced a zone of hydrolysis in starch-nutrient agar medium and generated various amylases in liquid medium. One isolate was selected as the best amylase producer and was identified as Bacillus licheniformis BT5.9. The improvement of culture conditions (initial medium pH of 5.0, cultivation temperature of 50°C, agitation speed of 100 rpm and inoculum size of 1.7 × 10(9) cells/ml) provided the highest amylase production (0.327 U/ml).
从印度尼西亚马朗的尚加尔温泉采集的土壤样本中总共获得了34株细菌分离株。其中，13株分离株在淀粉营养琼脂培养基中产生水解圈，并在液体培养基中产生各种淀粉酶。选择一株作为最佳淀粉酶产生菌，鉴定为地衣芽孢杆菌BT5.9。培养条件的优化（初始培养基pH为5.0、培养温度为50°C、搅拌速度为100 rpm、接种量为1.7×10⁹个细胞/ml）使淀粉酶产量最高（0.327 U/ml）。
