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嗜鞣管囊酵母木糖转运缺陷突变株的分离与鉴定。

Isolation and Characterization of Pichia heedii Mutants Defective in Xylose Uptake.

机构信息

Department of Viticulture and Enology, University of California, Davis, California 95616.

出版信息

Appl Environ Microbiol. 1990 Nov;56(11):3321-8. doi: 10.1128/aem.56.11.3321-3328.1990.

Abstract

To investigate the role of xylose uptake in xylose metabolism in yeasts, we isolated a series of mutated strains of the yeast Pichia heedii which are defective in xylose utilization. Four of these demonstrated defects in xylose uptake. Overlaps between the functional or regulatory mechanisms for glucose and xylose uptake may exist in this yeast since some of the mutants defective in xylose uptake were also defective in glucose transport. None of the mutants were defective in xylose reductase or xylitol dehydrogenase activities.

摘要

为了研究木糖摄取在酵母木糖代谢中的作用,我们分离了一系列酵母毕赤氏酵母中木糖利用缺陷的突变株。其中四种表现出木糖摄取缺陷。由于一些木糖摄取缺陷的突变体也存在葡萄糖转运缺陷,因此该酵母中葡萄糖和木糖摄取的功能或调节机制可能存在重叠。突变体中没有一种在木糖还原酶或木糖醇脱氢酶活性方面存在缺陷。

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