酵母希德氏毕赤酵母和酿酒酵母木糖摄取特性的研究。
Characterization of Xylose Uptake in the Yeasts Pichia heedii and Pichia stipitis.
机构信息
Department of Viticulture and Enology, University of California-Davis, Davis, California 95616.
出版信息
Appl Environ Microbiol. 1989 Jan;55(1):159-64. doi: 10.1128/aem.55.1.159-164.1989.
The kinetics of xylose uptake were investigated in the efficient xylose fermenter Pichia stipitis and in the more readily genetically manipulated, strictly respiratory yeast Pichia heedii. Both yeasts demonstrated more than one xylose uptake system, differing in substrate affinity. The K(m) of high-affinity xylose uptake in both organisms was similar to that of the efficient high-affinity glucose uptake system of Saccharomyces cerevisiae. In P. heedii, low-affinity xylose uptake was enhanced with growth on 2% but not 0.05% xylose and high-affinity uptake was reduced. In contrast to glucose uptake, xylose uptake in P. heedii was inhibited by dinitrophenol. Dinitrophenol inhibited both glucose and xylose uptake by P. stipitis. Glucose uptake was not inhibited by a 100-fold molar excess of xylose in P. heedii. It is suggested that xylose uptake in P. heedii is via a carrier system(s) distinct from those for glucose uptake.
木糖摄取的动力学在高效木糖发酵酵母毕赤酵母和更易于遗传操作的严格呼吸酵母汉逊毕赤酵母中进行了研究。这两种酵母都表现出不止一种木糖摄取系统，其底物亲和力不同。两种生物中高亲和力木糖摄取的 K(m)与酿酒酵母高效高亲和力葡萄糖摄取系统的 K(m)相似。在 P. heedii 中，低亲和力木糖摄取在以 2%但不是 0.05%木糖生长时增强，而高亲和力摄取减少。与葡萄糖摄取不同，二硝基苯酚抑制 P. heedii 中的木糖摄取。二硝基苯酚抑制 P. stipitis 中的葡萄糖和木糖摄取。在 P. heedii 中，100 倍摩尔过量的木糖不会抑制葡萄糖摄取。因此，推测 P. heedii 中的木糖摄取是通过不同于葡萄糖摄取的载体系统。
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