Laboratoire de Génie des Procédés Biotechnologiques Agro-Alimentaires, Centre de Biotechnologies Agro-Industrielles, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon, Rhône-Poulenc Santé, Centre de Recherche de Vitry-Alfortville, 94403 Vitry sur Seine, and Service de Microscopie, Centre National de la Recherche Agronomique, 78000 Versailles, France, and Nattermann Phospholipid GmbH, D-5000 Cologne, Federal Republic of Germany.
Appl Environ Microbiol. 1990 Dec;56(12):3811-6. doi: 10.1128/aem.56.12.3811-3816.1990.
Stimulation of lignin peroxidase production by exogenous phospholipids depends on the composition of the phospholipid fraction prepared by using the Nattermann process. The fraction composed mainly of negatively charged phospholipids (NAT 89) was the most efficient source for exoprotein secretion by Phanerochaete chrysosporium INA-12. The results of biochemical marker assays and ultrastructural morphology determination by electron microscopy were correlated. Activities of succinate dehydrogenase, a mitochondrial marker, and cytochrome c oxidoreductase, an endoplasmic reticulum (ER) marker, were increased 1.3- and 2.2-fold, respectively, in the presence of NAT 89. Electron microscopy observations suggested that the amount of mitochondria and ER in culture containing phospholipids was increased at the optimum day of lignin peroxidase production. Therefore, phospholipids enhanced energetic metabolism of strain INA-12 and markedly modified fungus physiology. Since ER is involved in enzyme synthesis, we suggest that its increased amount in mycelium cultured with NAT 89 is directly associated with the higher production of lignin peroxidase.
外源性磷脂对木质素过氧化物酶产生的刺激作用取决于采用 Nattermann 工艺制备的磷脂部分的组成。由带负电荷的磷脂组成的主要部分（NAT 89）是产黄青霉 INA-12 分泌外蛋白的最有效来源。通过电子显微镜进行的生物化学标记物测定和超微结构形态测定的结果相关联。琥珀酸脱氢酶（线粒体标记物）和细胞色素 c 氧化还原酶（内质网（ER）标记物）的活性分别增加了 1.3 倍和 2.2 倍。在含有磷脂的条件下，电子显微镜观察表明，在木质素过氧化物酶产生的最佳日子里，培养物中含有线粒体和 ER 的数量增加。因此，磷脂增强了 INA-12 菌株的能量代谢，并显著改变了真菌的生理学。由于 ER 参与酶的合成，因此我们建议，与在含有 NAT 89 的培养基中培养的菌丝体中 ER 数量的增加直接相关的是木质素过氧化物酶的产量更高。
