Soil Science Department, 2171 McCarty Hall, University of Florida, Gainesville, Florida 32611-0151.
Appl Environ Microbiol. 1992 Jan;58(1):229-32. doi: 10.1128/aem.58.1.229-232.1992.
For efficient handling, vesicular-arbuscular mycorrhizal fungi should be processed into small and uniform inocula; however, processing can reduce the inoculum density. In this article we describe the preparation and use of sheared-root inocula of Glomus spp. in which inoculum densities were increased during processing. Our objectives were to determine inoculum viability and density after shearing and to ascertain if the sheared inocula could be pelletized or used with a gel carrier. Root samples were harvested from aeroponic cultures, blotted dry, cut into 1-cm lengths, and sheared in a food processor for up to 80 s. After shearing, the inoculum was washed over sieves, and the propagule density in each fraction was determined. Sheared inocula were also encapsulated in carrageenan or used in a gel carrier. Shearing aeroponically produced root inocula reduced particle size. Propagule density increased with decreasing size fraction down to a size of 63 mum, after which propagule density decreased. The weighted-average propagule density of the inoculum was 135,380 propagules g (dry weight) of sheared root material. Sheared roots were encapsulated successfully in carrageenan, and the gel served as an effective carrier. Aeroponic root inoculum was stored dry at 4 degrees C for 23 months without significant reduction in propagule density; however, this material was not appropriate for shearing. Moist roots, useful for shearing, began to lose propagule density after 1 month of storage. Shearing proved to be an excellent method to prepare viable root inocula of small and uniform size, allowing for more efficient and effective use of limited inoculum supplies.
为了提高处理效率，泡囊丛枝菌应该被加工成小而均匀的接种体；然而，加工会降低接种体密度。本文描述了剪切根接种体的制备和应用，其中接种体密度在加工过程中增加。我们的目的是确定剪切后接种体的活力和密度，并确定剪切接种体是否可以制成颗粒或与凝胶载体一起使用。从气培培养物中收获根样本，擦干，切成 1 厘米长，在食品加工机中剪切长达 80 秒。剪切后，将接种体冲洗过筛，确定每个部分的繁殖体密度。剪切接种体也可以包封在卡拉胶中或与凝胶载体一起使用。气培生产的根接种体的剪切减小了颗粒尺寸。繁殖体密度随粒径减小而增加，直到粒径减小到 63 微米，之后繁殖体密度下降。接种体的加权平均繁殖体密度为 135380 个繁殖体 g（干重）剪切根材料。剪切根成功地包封在卡拉胶中，凝胶是一种有效的载体。气培根接种体在 4°C 下干燥储存 23 个月，繁殖体密度没有显著降低；然而，这种材料不适合剪切。湿根，可用于剪切，在储存 1 个月后开始失去繁殖体密度。剪切被证明是一种制备具有小而均匀尺寸的有活力的根接种体的极好方法，允许更有效地利用有限的接种体供应。
