荧光检测嗜热脂肪芽孢杆菌孢子结合酶α-D-葡萄糖苷酶，快速指示闪蒸灭菌失败。
Fluorimetric Detection of a Bacillus stearothermophilus Spore-Bound Enzyme, alpha-d-Glucosidase, for Rapid Indication of Flash Sterilization Failure.
机构信息
School of Public Health, Box 197 Mayo, University of Minnesota, Minneapolis, Minnesota 55455, and 3M Company, St. Paul, Minnesota 55133.
出版信息
Appl Environ Microbiol. 1992 Feb;58(2):717-9. doi: 10.1128/aem.58.2.717-719.1992.
Abstract
A biological indicator based on fluorimetric detection within 60 min of a Bacillus stearothermophilus spore-bound enzyme, alpha-d-glucosidase, has been developed. Results indicate that the enzyme survived slightly longer than spores observed after 24 h of incubation. The new system shows promise for evaluating flash sterilization cycles within 60 min compared with conventional 24-h systems.
摘要
一种基于荧光检测的生物指示剂已经被开发出来，该指示剂基于嗜热脂肪芽孢杆菌孢子结合酶（α-d-葡萄糖苷酶），检测时间在 60 分钟以内。结果表明，与 24 小时孵育后观察到的孢子相比，这种酶的存活时间稍长。与传统的 24 小时系统相比，该新系统有望在 60 分钟内评估快速灭菌循环。
相似文献
1
Fluorimetric Detection of a Bacillus stearothermophilus Spore-Bound Enzyme, alpha-d-Glucosidase, for Rapid Indication of Flash Sterilization Failure.荧光检测嗜热脂肪芽孢杆菌孢子结合酶α-D-葡萄糖苷酶，快速指示闪蒸灭菌失败。
Appl Environ Microbiol. 1992 Feb;58(2):717-9. doi: 10.1128/aem.58.2.717-719.1992.
2
Analysis of α-glucosidase enzyme activity used in a rapid test for steam sterilization assurance.用于蒸汽灭菌保证快速检测的α-葡萄糖苷酶活性分析。
J Appl Microbiol. 2016 May;120(5):1326-35. doi: 10.1111/jam.13074. Epub 2016 Mar 30.
3
Biological indicators for steam sterilization: characterization of a rapid biological indicator utilizing Bacillus stearothermophilus spore-associated alpha-glucosidase enzyme.蒸汽灭菌的生物指示剂：利用嗜热脂肪芽孢杆菌孢子相关α-葡萄糖苷酶的快速生物指示剂的特性
J Appl Microbiol. 1998 Nov;85(5):865-74. doi: 10.1046/j.1365-2672.1998.00607.x.
4
Effects of steam autoclave treatment on Geobacillus stearothermophilus spores.蒸汽高压灭菌处理对嗜热栖热放线菌芽孢的影响。
J Appl Microbiol. 2016 Nov;121(5):1300-1311. doi: 10.1111/jam.13257. Epub 2016 Oct 1.
5
Evaluation of a rapid readout biological indicator for flash sterilization with three biological indicators and three chemical indicators.使用三种生物指示剂和三种化学指示剂对快速灭菌的快速读数生物指示剂进行评估。
Infect Control Hosp Epidemiol. 1993 Jul;14(7):390-4. doi: 10.1086/646767.
6
An evaluation of three biological indicator systems in flash sterilization.三种生物指示剂系统在快速灭菌中的评估。
Infect Control. 1987 Aug;8(8):311-6. doi: 10.1017/s019594170006639x.
7
Use of biological indicators designed for steam or ethylene oxide to monitor a liquid chemical sterilization process.使用专为蒸汽或环氧乙烷设计的生物指示剂来监测液体化学消毒过程。
Infect Control Hosp Epidemiol. 1993 Jun;14(6):313-9. doi: 10.1086/646749.
8
9
Selection of biological indicator for validating microwave heating sterilization.用于验证微波加热灭菌的生物指示剂的选择。
PDA J Pharm Sci Technol. 1998 Mar-Apr;52(2):60-5.
10
Sporicidal efficacy of genipin: a potential theoretical alternative for biomaterial and tissue graft sterilization.京尼平的杀孢子效力：生物材料和组织移植物灭菌的一种潜在理论替代方法。
Cell Tissue Bank. 2013 Sep;14(3):381-93. doi: 10.1007/s10561-012-9335-z. Epub 2012 Aug 9.
引用本文的文献
1
Identification and characterization of new proteins crucial for bacterial spore resistance and germination.对细菌孢子抗性和萌发至关重要的新蛋白质的鉴定与表征
Front Microbiol. 2023 Apr 11;14:1161604. doi: 10.3389/fmicb.2023.1161604. eCollection 2023.
2
Application of Rapid Biological Indicators Coupled With Auto-Reader for the Quality Assurance of Surgical Instruments After Sterilization at a Cardiac Hospital in Bangladesh.快速生物指示剂结合自动阅读器在孟加拉国一家心脏医院手术器械灭菌后质量保证中的应用。
Cureus. 2021 Nov 10;13(11):e19428. doi: 10.7759/cureus.19428. eCollection 2021 Nov.
本文引用的文献
1
Biological indicators for steam sterilization--a U.S.P. collaborative study.蒸汽灭菌生物指示剂——美国药典协作研究
Bull Parenter Drug Assoc. 1972 Sep-Oct;26(5):205-11.
2
Flash sterilization. Evaluating biological indicators.快速灭菌。生物指示剂的评估。
AORN J. 1986 Jun;43(6):1289-90, 1292, 1294. doi: 10.1016/s0001-2092(07)65158-8.
3
An evaluation of three biological indicator systems in flash sterilization.三种生物指示剂系统在快速灭菌中的评估。
Infect Control. 1987 Aug;8(8):311-6. doi: 10.1017/s019594170006639x.
Zotero 插件