• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus.从嗜热古菌 Pyrococcus furiosus 中纯化和表征蛋白酶体。
Appl Environ Microbiol. 1997 Mar;63(3):1160-4. doi: 10.1128/aem.63.3.1160-1164.1997.
2
Purification and Characterization of Two Functional Forms of Intracellular Protease PfpI from the Hyperthermophilic Archaeon Pyrococcus furiosus.从嗜热古菌 Pyrococcus furiosus 中纯化和表征两种具有功能的胞内蛋白酶 PfpI 形式。
Appl Environ Microbiol. 1997 Jan;63(1):289-95. doi: 10.1128/aem.63.1.289-295.1997.
3
Purification and characterization of an extremely thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus.来自嗜热古菌激烈火球菌的一种极端耐热β-葡萄糖苷酶的纯化与特性分析
Eur J Biochem. 1993 Apr 1;213(1):305-12. doi: 10.1111/j.1432-1033.1993.tb17763.x.
4
Purification and characterization of a membrane-bound hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus.嗜热古菌激烈火球菌膜结合氢化酶的纯化与表征
J Bacteriol. 2000 Jun;182(12):3423-8. doi: 10.1128/JB.182.12.3423-3428.2000.
5
Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.β1亚基在嗜热古菌激烈火球菌20S蛋白酶体功能及稳定性中的作用
J Bacteriol. 2007 Jan;189(2):583-90. doi: 10.1128/JB.01382-06. Epub 2006 Nov 17.
6
Purification and characterization of two reversible and ADP-dependent acetyl coenzyme A synthetases from the hyperthermophilic archaeon Pyrococcus furiosus.从嗜热古菌激烈火球菌中纯化和鉴定两种可逆且依赖二磷酸腺苷的乙酰辅酶A合成酶
J Bacteriol. 1996 Oct;178(20):5897-903. doi: 10.1128/jb.178.20.5897-5903.1996.
7
Purification and characterization of the alanine aminotransferase from the hyperthermophilic Archaeon pyrococcus furiosus and its role in alanine production.嗜热古菌激烈火球菌丙氨酸转氨酶的纯化、特性鉴定及其在丙氨酸生产中的作用
J Bacteriol. 2000 May;182(9):2559-66. doi: 10.1128/JB.182.9.2559-2566.2000.
8
Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus: substrate specificity and primary structure analysis.嗜热古菌激烈火球菌5'-甲硫腺苷磷酸化酶的纯化与特性：底物特异性及一级结构分析
Extremophiles. 2003 Apr;7(2):159-68. doi: 10.1007/s00792-002-0307-2. Epub 2003 Jan 10.
9
Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus.嗜热古菌激烈火球菌中一种新型细胞内蛋白酶（PfpI）编码基因的序列、在大肠杆菌中的表达及分析
J Bacteriol. 1996 May;178(9):2605-12. doi: 10.1128/jb.178.9.2605-2612.1996.
10
S-adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: biochemical characterization and analysis of protein structure by comparative molecular modeling.嗜热栖热菌来源的S-腺苷高半胱氨酸水解酶：生物化学特性及通过比较分子建模对蛋白质结构的分析
Proteins. 2005 Mar 1;58(4):815-25. doi: 10.1002/prot.20381.

引用本文的文献

1
Interplay between transcriptional regulators and VapBC toxin-antitoxin loci during thermal stress response in extremely thermoacidophilic archaea.在极端嗜热嗜酸古菌的热应激反应中，转录调控因子与 VapBC 毒素-抗毒素基因座之间的相互作用。
Environ Microbiol. 2023 Jun;25(6):1200-1215. doi: 10.1111/1462-2920.16350. Epub 2023 Feb 24.
2
Proteolytic systems of archaea: slicing, dicing, and mincing in the extreme.古细菌的蛋白水解系统：在极端环境下的切割、切块和切碎
Emerg Top Life Sci. 2018 Dec;2(4):561-580. doi: 10.1042/etls20180025. Epub 2018 Nov 14.
3
Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms.极端嗜热微生物的生理、代谢及生物技术特性
Wiley Interdiscip Rev Syst Biol Med. 2017 May;9(3). doi: 10.1002/wsbm.1377. Epub 2017 Feb 16.
4
Archaeal proteasomes and sampylation.古菌蛋白酶体与泛素化修饰
Subcell Biochem. 2013;66:297-327. doi: 10.1007/978-94-007-5940-4_11.
5
Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.β1亚基在嗜热古菌激烈火球菌20S蛋白酶体功能及稳定性中的作用
J Bacteriol. 2007 Jan;189(2):583-90. doi: 10.1128/JB.01382-06. Epub 2006 Nov 17.
6
Heat shock response by the hyperthermophilic archaeon Pyrococcus furiosus.嗜热古菌激烈火球菌的热休克反应
Appl Environ Microbiol. 2003 Apr;69(4):2365-71. doi: 10.1128/AEM.69.4.2365-2371.2003.
7
Chemical denaturation and elevated folding temperatures are required for wild-type activity and stability of recombinant Methanococcus jannaschii 20S proteasome.重组詹氏甲烷球菌20S蛋白酶体的野生型活性和稳定性需要化学变性和提高折叠温度。
Protein Sci. 2001 Sep;10(9):1887-96. doi: 10.1110/ps.ps.05801.
8
Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome.詹氏甲烷球菌20S蛋白酶体和PAN（真核生物26S蛋白酶体ATP酶（Rpt）亚基的同源物）的生化和物理特性。
J Bacteriol. 2000 Mar;182(6):1680-92. doi: 10.1128/JB.182.6.1680-1692.2000.
9
Halophilic 20S proteasomes of the archaeon Haloferax volcanii: purification, characterization, and gene sequence analysis.嗜盐古菌沃氏嗜盐碱杆菌的嗜盐20S蛋白酶体：纯化、表征及基因序列分析
J Bacteriol. 1999 Sep;181(18):5814-24. doi: 10.1128/JB.181.18.5814-5824.1999.
10
The 20S proteasome of Streptomyces coelicolor.天蓝色链霉菌的20S蛋白酶体。
J Bacteriol. 1998 Oct;180(20):5448-53. doi: 10.1128/JB.180.20.5448-5453.1998.

本文引用的文献

1
Purification and Characterization of Two Functional Forms of Intracellular Protease PfpI from the Hyperthermophilic Archaeon Pyrococcus furiosus.从嗜热古菌 Pyrococcus furiosus 中纯化和表征两种具有功能的胞内蛋白酶 PfpI 形式。
Appl Environ Microbiol. 1997 Jan;63(1):289-95. doi: 10.1128/aem.63.1.289-295.1997.
2
Regulation of Proteolytic Activity in the Hyperthermophile Pyrococcus furiosus.在嗜热古菌 Pyrococcus furiosus 中调节蛋白水解活性。
Appl Environ Microbiol. 1992 Apr;58(4):1134-41. doi: 10.1128/aem.58.4.1134-1141.1992.
3
Characterization of Amylolytic Enzyme Activities Associated with the Hyperthermophilic Archaebacterium Pyrococcus furiosus.与嗜热古菌 Pyrococcus furiosus 相关的淀粉酶活性的表征。
Appl Environ Microbiol. 1990 Jul;56(7):1985-91. doi: 10.1128/aem.56.7.1985-1991.1990.
4
Isolation and characterization of the hyperthermostable serine protease, pyrolysin, and its gene from the hyperthermophilic archaeon Pyrococcus furiosus.从嗜热古菌激烈火球菌中分离并鉴定超嗜热丝氨酸蛋白酶热解素及其基因。
J Biol Chem. 1996 Aug 23;271(34):20426-31. doi: 10.1074/jbc.271.34.20426.
5
Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.产甲烷古菌詹氏甲烷球菌的全基因组序列
Science. 1996 Aug 23;273(5278):1058-73. doi: 10.1126/science.273.5278.1058.
6
Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus.嗜热古菌激烈火球菌中一种新型细胞内蛋白酶（PfpI）编码基因的序列、在大肠杆菌中的表达及分析
J Bacteriol. 1996 May;178(9):2605-12. doi: 10.1128/jb.178.9.2605-2612.1996.
7
Purification and characterization of an extremely thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus.来自嗜热古菌激烈火球菌的一种极端耐热β-葡萄糖苷酶的纯化与特性分析
Eur J Biochem. 1993 Apr 1;213(1):305-12. doi: 10.1111/j.1432-1033.1993.tb17763.x.
8
The multicatalytic and 26 S proteases.多催化蛋白酶和26S蛋白酶。
J Biol Chem. 1993 Mar 25;268(9):6065-8.
9
Purification and characterization of the 26S proteasome complex catalyzing ATP-dependent breakdown of ubiquitin-ligated proteins from rat liver.大鼠肝脏中催化泛素连接蛋白ATP依赖性降解的26S蛋白酶体复合物的纯化与鉴定
J Biochem. 1993 Jun;113(6):754-68. doi: 10.1093/oxfordjournals.jbchem.a124116.
10
Dissociation and reconstitution of the Thermoplasma proteasome.嗜热栖热菌蛋白酶体的解离与重组
Eur J Biochem. 1994 Aug 1;223(3):1061-7. doi: 10.1111/j.1432-1033.1994.tb19084.x.

从嗜热古菌 Pyrococcus furiosus 中纯化和表征蛋白酶体。

Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus.

出版信息

Appl Environ Microbiol. 1997 Mar;63(3):1160-4. doi: 10.1128/aem.63.3.1160-1164.1997.

DOI:10.1128/aem.63.3.1160-1164.1997
PMID:16535545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1389139/
Abstract

A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.

摘要

一种由（α）（25kDa）和（β）（22kDa）亚基组成的 640kDa 蛋白酶体，最适温度为 95°C，从嗜热古菌 Pyrococcus furiosus 的粗细胞提取物中纯化得到。尽管这是第四个被发现含有蛋白酶体的真核生物界（第一个嗜热菌）成员，但在古菌界成员中尚未发现。