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从嗜热古菌 Pyrococcus furiosus 中纯化和表征蛋白酶体。

Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus.

出版信息

Appl Environ Microbiol. 1997 Mar;63(3):1160-4. doi: 10.1128/aem.63.3.1160-1164.1997.

Abstract

A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.

摘要

一种由(α)(25kDa)和(β)(22kDa)亚基组成的 640kDa 蛋白酶体,最适温度为 95°C,从嗜热古菌 Pyrococcus furiosus 的粗细胞提取物中纯化得到。尽管这是第四个被发现含有蛋白酶体的真核生物界(第一个嗜热菌)成员,但在古菌界成员中尚未发现。

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