Toriyama S, Guy P L, Fuji S, Takahashi M
National Institute of Agro-Environmental Sciences, Ibaraki, Japan.
J Gen Virol. 1992 Apr;73 ( Pt 4):1021-3. doi: 10.1099/0022-1317-73-4-1021.
Picorna-like virus particles, 29 nm in diameter, were purified from apparently healthy Laodelphax striatellus Fallen. The virus particles had a buoyant density of 1.352 g/ml in CsCl and a sedimentation coefficient of 161 s. The virus capsid proteins consisted of three major polypeptides of M(r)s 36,500, 33,000 and 28,000, and three minor polypeptides. The virus contained a major ssRNA of M(r) 2.8 x 10(6) and was also frequently associated with a minor dsRNA of M(r) 4 x 10(6). The 3' end of the ssRNA had a poly(A) tract of about 60 adenine residues. The virus has been provisionally named himetobi P virus.
从表面健康的灰飞虱中纯化出直径为29纳米的类微小核糖核酸病毒颗粒。这些病毒颗粒在氯化铯中的浮力密度为1.352克/毫升，沉降系数为161 s。病毒衣壳蛋白由分子量分别为36,500、33,000和28,000的三种主要多肽以及三种次要多肽组成。该病毒含有一条分子量为2.8×10⁶的主要单链核糖核酸，并且还经常与一条分子量为4×10⁶的次要双链核糖核酸相关联。单链核糖核酸的3'端有一段约60个腺嘌呤残基的聚腺苷酸序列。该病毒已被暂时命名为灰飞虱P病毒。
