Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agricultural Sciences, Nagoya University, Nagoya 464-01, Japan.
Appl Environ Microbiol. 1992 Nov;58(11):3574-9. doi: 10.1128/aem.58.11.3574-3579.1992.
Strains of two types of methylotrophic bacteria, Paracoccus denitrificans and Methylobacterium extorquens, synthesized the copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) when methanol and n-amyl alcohol were added together to nitrogen-limited medium. The composition of the copolyester differed considerably between the two strains: the copolyester from P. denitrificans was comparatively rich in 3-hydroxyvalerate (3HV). The 3HV content of the copolyester synthesized by this strain increased with increasing concentrations of n-amyl alcohol. Its maximum content was 91.5 mol% under the conditions used. In M. extorquens, the maximum 3HV content was limited to 38.2 mol%. Since n-amyl alcohol served as a substrate for a standard methanol dehydrogenase, the enzyme was proposed to oxidize both methanol and n-amyl alcohol in the first step of copolyester synthesis from these substrates by methanol-grown cells.
两种甲醇营养型细菌，即脱氮副球菌和扩展甲基杆菌，在氮限制培养基中添加甲醇和正戊醇时，可以合成共聚酯聚（3-羟基丁酸-co-3-羟基戊酸酯）。两种菌株合成的共聚酯的组成有很大差异：脱氮副球菌的共聚酯中 3-羟基戊酸（3HV）含量较高。该菌株合成的共聚酯的 3HV 含量随正戊醇浓度的增加而增加。在使用的条件下，其最大含量为 91.5mol%。在扩展甲基杆菌中，3HV 的最大含量限制为 38.2mol%。由于正戊醇是标准甲醇脱氢酶的底物，因此该酶被认为可以在甲醇生长细胞合成共聚酯的第一步中氧化甲醇和正戊醇这两种底物。
