Department of Forest Products, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan.
Appl Environ Microbiol. 1993 Feb;59(2):438-41. doi: 10.1128/aem.59.2.438-441.1993.
When 4-methylguaiacol (MeG), a phenolic lignin model compound, was added to a culture that was inoculated with Coriolus versicolor, it was bioconverted into 2-methoxy-4-methylphenyl beta-d-xyloside (MeG-Xyl). The phenolic hydroxyl group of vanillyl alcohol was much more extensively xylosylated than the alcoholic hydroxyl group. When a mixture of MeG and commercial UDP-xylose was incubated with cell extracts of mycelia, transformation of UDP-xylose into MeG-Xyl was observed. This result suggested that UDP-xylosyltransferase was involved in the xylosylation of phenolic hydroxyl groups of lignin model compounds.
当 4-甲基愈创木酚(MeG),一种酚类木质素模型化合物,被添加到接种了绒盖牛肝菌的培养物中时,它被生物转化为 2-甲氧基-4-甲基苯-β-D-木糖苷(MeG-Xyl)。香草醇的酚羟基比醇羟基更广泛地发生了木糖基化。当 MeG 和商业 UDP-木糖的混合物与菌丝体的细胞提取物一起孵育时,观察到 UDP-木糖转化为 MeG-Xyl。这一结果表明 UDP-木糖苷基转移酶参与了木质素模型化合物酚羟基的木糖基化。