Bourbonnais R, Paice M G
Pulp and Paper Research Institute of Canada, Pointe Claire, Que.
FEBS Lett. 1990 Jul 2;267(1):99-102. doi: 10.1016/0014-5793(90)80298-w.
In the presence of substrates such as Remazol Blue and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate) (ABTS), laccases Coriolus (Trametes) versicolor can also oxidize non-phenolic lignin model compounds. Veratryl alcohol (I) and 1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-propane-1,3-diol (III) were oxidized by laccase and mediator to give the alpha-carbonyl derivatives. The beta-1 lignin model dimer, 1-(3,4-dimethoxyphenyl)-2-phenoxy-ethane-1,2-diol (II) was cleaved by laccase in the presence of ABTS to give veratraldehyde and benzaldehyde. On the basis of these observations, we propose that laccase is capable of oxidizing both phenolic and non-phenolic moieties of lignin but that the latter is dependent on the co-presence of primary laccase substrates.
在存在诸如雷马素蓝和2,2'-偶氮双(3-乙基苯并噻唑啉-6-磺酸盐)(ABTS)等底物的情况下，云芝漆酶也能够氧化非酚类木质素模型化合物。藜芦醇(I)和1-(3,4-二甲氧基苯基)-2-(2-甲氧基苯氧基)-丙烷-1,3-二醇(III)被漆酶和介体氧化生成α-羰基衍生物。β-1木质素模型二聚体1-(3,4-二甲氧基苯基)-2-苯氧基-乙烷-1,2-二醇(II)在ABTS存在下被漆酶裂解生成藜芦醛和苯甲醛。基于这些观察结果，我们提出漆酶能够氧化木质素的酚类和非酚类部分，但后者依赖于主要漆酶底物的共同存在。
