Sutherland J B, Selby A L, Freeman J P, Evans F E, Cerniglia C E
National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.
Appl Environ Microbiol. 1991 Nov;57(11):3310-6. doi: 10.1128/aem.57.11.3310-3316.1991.
The white rot fungus Phanerochaete chrysosporium metabolized phenanthrene when it was grown for 7 days at 37 degrees C in a medium containing malt extract, D-glucose, D-maltose, yeast extract, and Tween 80. After cultures were grown with [9-14C]phenanthrene, radioactive metabolites were extracted from the medium with ethyl acetate, separated by high-performance liquid chromatography, and detected by liquid scintillation counting. Metabolites from cultures grown with unlabeled phenanthrene were identified as phenanthrene trans-9,10-dihydrodiol, phenanthrene trans-3,4-dihydrodiol, 9-phenanthrol, 3-phenanthrol, 4-phenanthrol, and the novel conjugate 9-phenanthryl beta-D-glucopyranoside. Identification of the compounds was based on their UV absorption, mass, and nuclear magnetic resonance spectra. Since lignin peroxidase was not detected in the culture medium, these results suggest the involvement of monooxygenase and epoxide hydrolase activity in the initial oxidation and hydration of phenanthrene by P. chrysosporium.
在含有麦芽提取物、D-葡萄糖、D-麦芽糖、酵母提取物和吐温80的培养基中，白腐真菌黄孢原毛平革菌在37℃下培养7天时可代谢菲。用[9-¹⁴C]菲培养后，用乙酸乙酯从培养基中提取放射性代谢物，通过高效液相色谱分离，并用液体闪烁计数法检测。用未标记菲培养的代谢物被鉴定为菲反式-9,10-二氢二醇、菲反式-3,4-二氢二醇、9-菲醇、3-菲醇、4-菲醇以及新型共轭物9-菲基-β-D-吡喃葡萄糖苷。化合物的鉴定基于其紫外吸收、质谱和核磁共振光谱。由于在培养基中未检测到木质素过氧化物酶，这些结果表明单加氧酶和环氧化物水解酶活性参与了黄孢原毛平革菌对菲的初始氧化和水合作用。
