Department of Plant Pathology, University of California, Riverside, California 92521-0122.
Appl Environ Microbiol. 1993 May;59(5):1671-4. doi: 10.1128/aem.59.5.1671-1674.1993.
Copper resistance in Pseudomonas syringae carrying the copABCD operon is associated with accumulation of copper in the periplasm and outer membrane, apparently as a function of the copper-binding activities of the copABC gene products. However, no specific function for copD has been determined. In this study, P. syringae cells containing copCD or copBCD cloned behind the lac promoter were hypersensitive to copper. An increased accumulation of copper was measured in cells containing several combinations of cop genes that included copC and copD. Our data suggest that CopC, a periplasmic copper-binding protein, and CopD, a probable inner membrane protein, may function together in copper uptake.
铜在携带 copABCD 操纵子的丁香假单胞菌中的抗性与铜在周质和外膜中的积累有关,显然是 copABC 基因产物的铜结合活性的功能。然而,copD 的具体功能尚未确定。在这项研究中,含有 copCD 或 copBCD 克隆在 lac 启动子之后的丁香假单胞菌细胞对铜敏感。在包含包括 copC 和 copD 的几种 cop 基因组合的细胞中测量到铜的积累增加。我们的数据表明,周质铜结合蛋白 CopC 和可能的内膜蛋白 CopD 可能共同参与铜摄取。