Center of Marine Biotechnology, Maryland Biotechnology Institute, University of Maryland, 600 East Lombard Street, Baltimore, Maryland 21202.
Appl Environ Microbiol. 1993 Oct;59(10):3406-10. doi: 10.1128/aem.59.10.3406-3410.1993.
The epibenthic bacterial community at deep-ocean sewage sludge disposal site DWD-106, located approximately 106 miles (ca. 196 km) off the coast of New Jersey, was assessed for changes associated with the introduction of large amounts of sewage sludge. Mixed cultures and bacterial isolates obtained from water overlying sediment core samples collected at the deep-water (2,500 m) municipal sewage disposal site were tested for the ability to grow under in situ conditions of temperature and pressure. The responses of cultures collected at a DWD-106 station heavily impacted by sewage sludge were compared with those of samples collected from a station at the same depth which was not contaminated by sewage sludge. Significant differences were observed in the ability of mixed bacterial cultures and isolates from the two sites to grow under deep-sea pressure and temperature conditions. The levels of sludge contamination were established by enumerating Clostridium perfringens, a sewage indicator bacterium, in sediment samples from the two sites. The results of hybridization experiments in which DNAs extracted directly from the water overlying sediment core samples were used indicate that the reference site epibenthic community, the disposal site epibenthic community, and the community in a surface sludge plume share many members. Decreased culturability of reference site mixed cultures in the presence of sewage sludge was observed. Thus, the culturable portions of both the autochthonous and allochthonous bacterial communities at the disposal site may be inhibited in situ, the former by sewage sludge and the latter by high pressure and low temperature.
位于新泽西州海岸约 106 英里（约合 196 公里）处的深海污水污泥处理场 DWD-106 的底栖细菌群落，因大量污水污泥的引入而发生了变化。对从位于深水区（2500 米）的城市污水处置场采集的沉积物芯水样上方的水获得的混合培养物和细菌分离物进行了测试，以评估其在原位温度和压力条件下的生长能力。对来自 DWD-106 站（该站受到污水污泥的严重影响）的培养物的响应与从同一深度的未受污水污泥污染的站采集的样品的响应进行了比较。从两个地点采集的混合细菌培养物和分离物在深海压力和温度条件下生长的能力存在明显差异。通过对两个地点的沉积物样本中产气荚膜梭菌（一种污水指示菌）的计数来确定污泥污染水平。直接从水上方的沉积物芯样本中提取 DNA 进行杂交实验的结果表明，参考点底栖群落、处置点底栖群落和表层污泥羽流中的群落有许多共同成员。在存在污水污泥的情况下，参考点混合培养物的可培养性降低。因此，处置场的自生和异养细菌群落的可培养部分可能在原位受到抑制，前者受污水污泥的抑制，后者受高压和低温的抑制。
