Research Station, Agriculture Canada, Beaverlodge, Alberta, Canada TOH OCO.
Appl Environ Microbiol. 1994 Feb;60(2):654-61. doi: 10.1128/aem.60.2.654-661.1994.
A panel of 11 monoclonal antibodies raised against vegetative cells of Rhizobium leguminosarum biovar trifolii or Rhizobium meliloti was tested by enzyme-linked immunosorbent assay for reactivity with 47 strains of R. leguminosarum biovar trifolii and 60 strains of R. meliloti. The goal of the study was to define the degree of specificity associated with each antibody and to gain an understanding of the amount of antigenic diversity found among the strains and between the species. Each antibody was tested against each Rhizobium strain in four forms: washed steamed cells, washed unsteamed cells, cell-free culture broth, and nodule squash material. Each antibody showed a different pattern of reactivity among the 107 strains. One of each of the antibodies developed against R. meliloti and R. leguminosarum biovar trifolii reacted in a highly specific manner with cells or antigen from the immunogenic strain only. Nine of the antibodies recognized secreted as well as cellular antigen from many of the strains. Analysis of patterns of reactivity between the 107 strains and the 11 antibodies separated the strains into 28 groups of which 12 were represented by one strain only.
一组针对根瘤菌属三型植物细胞或根瘤菌属 meliloti 的 11 种单克隆抗体通过酶联免疫吸附试验与 47 株根瘤菌属三型植物和 60 株根瘤菌属 meliloti 进行了反应性测试。该研究的目的是确定与每种抗体相关的特异性程度，并了解菌株之间以及种间抗原多样性的程度。每种抗体都以四种形式测试了每一种 Rhizobium 菌株：清洗蒸汽细胞、清洗未蒸汽细胞、无细胞培养肉汤和根瘤压碎材料。每种抗体在 107 株菌株中的反应模式都不同。针对根瘤菌属 meliloti 和根瘤菌属三型植物的每种抗体中的一种以高度特异性的方式与免疫原性菌株的细胞或抗原反应。9 种抗体识别许多菌株的分泌和细胞抗原。107 株菌株和 11 种抗体之间的反应性模式分析将菌株分为 28 组，其中 12 组仅由一株菌株代表。
