Kannenberg E L, Brewin N J
John Innes Institute, Norwich, United Kingdom.
J Bacteriol. 1989 Sep;171(9):4543-8. doi: 10.1128/jb.171.9.4543-4548.1989.
Rhizobium leguminosarum bv. viciae 3841 was grown in liquid suspension culture to investigate how culture conditions could affect the expression of a developmentally regulated cell surface antigen associated with lipopolysaccharide. The antigen, which is recognized by monoclonal antibody AFRC MAC 203, was expressed when cultures were grown at neutral pH under low-oxygen conditions (less than 7.5% [vol/vol] O2 in the gas phase). Antigen was also expressed in aerobically grown cultures at pH values below 5.3. The nature of the nitrogen and the carbon sources had no effect on antigen expression except by indirect changes on the pH of the culture medium; similarly, growth in 0.3 M NaCl did not result in antigen expression. The induction of MAC 203 antigen by low-oxygen or low-pH culture conditions is discussed in the context of tissue-specific expression within the legume root nodule.
豌豆根瘤菌蚕豆生物变种3841在液体悬浮培养中生长，以研究培养条件如何影响与脂多糖相关的发育调控细胞表面抗原的表达。该抗原可被单克隆抗体AFRC MAC 203识别，当培养物在中性pH值和低氧条件下（气相中氧气含量低于7.5%[体积/体积]）生长时表达。在pH值低于5.3的需氧培养物中也表达抗原。氮源和碳源的性质对抗原表达没有影响，除非对培养基的pH值有间接影响；同样，在0.3M NaCl中生长也不会导致抗原表达。在豆科植物根瘤内组织特异性表达的背景下，讨论了低氧或低pH培养条件对MAC 203抗原的诱导作用。
