Departmento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, La Laguna, Tenerife, Spain.
Appl Environ Microbiol. 1994 Aug;60(8):2971-6. doi: 10.1128/aem.60.8.2971-2976.1994.
A strain of Penicillium chrysogenum has been isolated from pine forest soils in Tenerife (Canary Islands). This strain was capable of utilizing hydroxylated and nonhydroxylated aromatic compounds, in particular cinnamic acid, as its sole carbon source. In an optimum medium with high levels of nitrogen (25.6 mM) and low levels of glucose (5.5 mM), it was able to decolorize Poly B-411 and to transform kraft, organosolv, and synthetic dehydrogenative polymerisate lignins. After 30 days of incubation, the amount of recovered kraft lignin was reduced to 83.5 and 91.3% of that estimated for uninoculated controls by spectrophotometry and klason lignin, respectively. At the same time, the pattern of molecular mass distribution of the lignin remaining in cultures was changed. The amount of organosolv lignin recovered from cultures was reduced to 90.1 and 94.6% of the initial amount as evaluated by spectrophotometry and klason lignin, respectively. About 6% of total applied radioactivity of OCH(3)-organosolv lignin was recovered as CO(2) after 30 days of incubation, and 18.5% of radioactivity from insoluble OCH(3)-organosolv lignin was solubilized. After 26 days of incubation, 2.9% of C-beta-dehydrogenative polymerisate and 4.1% of C-ring-dehydrogenative polymerisate evolved as CO(2).
已从特内里费（加那利群岛）松林土壤中分离到一株产黄青霉（Penicillium chrysogenum）。该菌株能够利用羟基化和非羟基化的芳香族化合物，特别是肉桂酸作为其唯一的碳源。在含有高浓度氮（25.6 mM）和低浓度葡萄糖（5.5 mM）的最佳培养基中，它能够使 Poly B-411 脱色，并转化 kraft、有机溶剂法和合成脱氢聚合物木质素。在 30 天的培养期后，分光光度法和 klason 木质素分别将未接种对照的 kraft 木质素的回收量减少到 83.5%和 91.3%。同时，培养物中木质素的分子量分布模式发生了变化。分光光度法和 klason 木质素分别将回收的有机溶剂法木质素的量减少到初始量的 90.1%和 94.6%。在 30 天的培养期后，约 6%的 OCH（3）-有机溶剂法木质素的总放射性活性以 CO（2）的形式回收，而不溶性 OCH（3）-有机溶剂法木质素的 18.5%的放射性活性被溶解。培养 26 天后，2.9%的 C-β-脱氢聚合物和 4.1%的 C-环-脱氢聚合物作为 CO（2）释放。
