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Optical coherence tomography: a new high-resolution imaging technology to study cardiac development in chick embryos.光学相干断层扫描：一种用于研究鸡胚心脏发育的新型高分辨率成像技术。
Circulation. 2002 Nov 26;106(22):2771-4. doi: 10.1161/01.cir.0000042672.51054.7b.
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Optical projection tomography as a tool for 3D microscopy and gene expression studies.光学投影断层成像作为一种用于三维显微镜检查和基因表达研究的工具。
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Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.生物组织中内源性结构蛋白的三维高分辨率二次谐波生成成像
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Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographs.通过电子显微镜照片的傅里叶合成对球形病毒进行三维重建的方法。
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一种用于生物组织切片图像的三维重建分割与分析的新技术。

A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues.

作者信息

Li Jing, Zhao Hai-yan, Ruan Xing-yun, Xu Yong-qing, Meng Wei-zheng, Li Kun-peng, Zhang Jing-qiang

机构信息

Department of Equipments, Kunming General Hospital, Kunming 650032, China.

出版信息

J Zhejiang Univ Sci B. 2005 Dec;6(12):1210-2. doi: 10.1631/jzus.2005.B1210.

DOI:10.1631/jzus.2005.B1210
PMID:16358381
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1390646/
Abstract

A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fourier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform simultaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction segmentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.

摘要

一种用于对包括通过去卷积方法进行的微观宽场光学切片、通过傅里叶 - 贝塞尔合成和电子断层扫描（ET）的冷冻电子显微镜切片以及一系列计算机断层扫描（CT）在内的图像序列切片进行三维（3D）重建、分割、显示和分析的新技术被开发出来，用于对生物医学样本的结构和功能进行同步测量。本文展示了花粉孢子、伴侣蛋白、病毒、头部、颈椎骨、胫骨和腕骨的3D重建分割显示和分析结果。同时，还提出了这项新技术在生物医学领域的一些潜在应用。