Shamekh R, Newcomb J, Mallery J, Cassady C J, Saporta S, Cameron D F, Sanberg P R, Willing A E
Center of Excellence for Aging & Brain Repair, University of South Florida, College of Medicine, Tampa 33612, USA.
Cell Transplant. 2005;14(8):551-64. doi: 10.3727/000000005783982747.
Transplanting cells across species (xenotransplantation) for the treatment of Parkinson's disease has been considered an option to alleviate ethical concerns and shortage of tissues. However, using this approach leads to decreased cell survival; the xenografted cells are often rejected. Sertoli cells (SCs) are testis-derived cells that provide immunological protection to developing germ cells and can enhance survival of both allografted and xenografted cells. It is not clear whether these cells will maintain their immunosuppressive support of cografted cells if they are transplanted across species. In this study, we investigated the immune modulatory capacity of SCs and the feasibility of xenografting these cells alone or with allografted and xenografted neural tissue. Transplanting xenografts of rat SCs into the mouse striatum with either rat or mouse ventral mesencephalon prevented astrocytic infiltration of the graft site, although all transplants showed activated microglia within the core of the graft. Surviving tyrosine hydroxylase-positive neurons were observed in all conditions, but the size of the grafts was small at best. SCs were found at 1 and 2 weeks posttransplant. However, few SCs were found at 2 months posttransplant. Further investigation is under way to characterize the immune capabilities of SCs in a xenogeneic environment.
跨物种移植细胞（异种移植）用于治疗帕金森病被认为是一种缓解伦理问题和组织短缺的选择。然而，使用这种方法会导致细胞存活率降低；异种移植的细胞常常被排斥。支持细胞（SCs）是来源于睾丸的细胞，能为发育中的生殖细胞提供免疫保护，并可提高同种移植和异种移植细胞的存活率。尚不清楚这些细胞跨物种移植后是否会对共移植的细胞维持其免疫抑制支持作用。在本研究中，我们调查了支持细胞的免疫调节能力以及单独移植这些细胞或与同种移植和异种移植神经组织一起移植的可行性。将大鼠支持细胞的异种移植物与大鼠或小鼠腹侧中脑一起移植到小鼠纹状体中，可防止移植部位的星形细胞浸润，尽管所有移植在移植物核心内均显示有活化的小胶质细胞。在所有情况下均观察到存活的酪氨酸羟化酶阳性神经元，但移植物的大小充其量很小。移植后1周和2周发现有支持细胞。然而，移植后2个月发现的支持细胞很少。正在进行进一步研究以表征支持细胞在异种环境中的免疫能力。
