Nagy Peter D, Pogany Judit
Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA.
Virology. 2006 Jan 5;344(1):211-20. doi: 10.1016/j.virol.2005.09.017.
RNA replication is the central process during the infectious cycles of plus-stranded RNA viruses. Development of yeast as a model host and powerful in vitro assays with purified replicase complexes, together with reverse genetic approaches make tombusviruses, small plant RNA viruses, excellent systems to study fundamental aspects of viral RNA replication. Accordingly, in vitro approaches have led to the identification of protein-RNA interactions that are essential for template selection for replication and assembly of the functional viral replicase complexes. Moreover, genome-wide approaches and proteomics analyses have identified a new set of host proteins that affected tombusvirus replication. Overall, rapid progress in tombusvirus replication has revealed intriguing and complex nature of virus-host interactions, which make robust replication of tombusviruses possible. The knowledge obtained will likely stimulate development of new antiviral methods as well as other approaches that could make tombusviruses useful tools in biotechnological applications.
RNA复制是正链RNA病毒感染周期中的核心过程。酵母作为一种模式宿主的发展以及利用纯化的复制酶复合物进行的强大体外分析，再加上反向遗传学方法，使得番茄丛矮病毒（tombusviruses）这种小型植物RNA病毒成为研究病毒RNA复制基本方面的优秀系统。因此，体外方法已导致鉴定出对于复制模板选择以及功能性病毒复制酶复合物组装至关重要的蛋白质-RNA相互作用。此外，全基因组方法和蛋白质组学分析已鉴定出一组影响番茄丛矮病毒复制的新宿主蛋白。总体而言，番茄丛矮病毒复制方面的快速进展揭示了病毒-宿主相互作用的有趣而复杂的本质，这使得番茄丛矮病毒能够进行强劲复制。所获得的知识可能会刺激新抗病毒方法以及其他方法的发展，这些方法可使番茄丛矮病毒成为生物技术应用中的有用工具。
