来自巴西患有局灶性脑病变且无占位效应的艾滋病患者脑脊液样本中的JC病毒DNA。

JC virus DNA in cerebrospinal fluid samples from Brazilian AIDS patients with focal brain lesions without mass effect.

作者信息

Fink M C D S, Penalva de Oliveira A C, Milagres F A P, Vidal J E, Picerno-Pouza A F, Duarte Neto A, Pannuti C S

机构信息

Laboratório de Virologia (LIM HC-FMUSP), Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Rua Dr Enéas de Carvalho Aguiar, 470, São Paulo, Brazil.

出版信息

J Infect. 2006 Jan;52(1):30-6. doi: 10.1016/j.jinf.2005.02.015.

DOI:10.1016/j.jinf.2005.02.015

PMID:16368458

Abstract

OBJECTIVE

To evaluate the presence of JC virus DNA in CSF samples from Brazilian AIDS patients with focal lesions of CNS white matter without mass effect compatible with progressive multifocal leukoencephalopathy (PML).

METHODS

CSF samples from AIDS patients with neurological symptoms and a CT scan showing focal lesions of CNS white matter without mass effect suggestive of PML, and from AIDS and non-AIDS patients with non-PML neurological diseases were tested for JC virus DNA by PCR. The primers used to amplify the T antigen region of the JC virus resulted in a 173-bp fragment. The presence of the JC virus was confirmed by digestion of the PCR product using BamH1.

RESULTS

The PCR for JCV DNA was negative in 119/120 non-PML CSF samples (specificity =99.2%). Of 56 CSF samples from AIDS patients with focal lesions of CNS white matter without mass effect, JCV DNA was positive in 48.2% (27/56). In 23/29 (79.3%) JCV DNA-negative cases, other causes for the encephalitic lesions were found. No JCV DNA-positive cases showed other diagnoses.

CONCLUSIONS

The prevalence of JCV DNA by PCR in CSF samples from Brazilian AIDS patients with focal brain lesions, without mass effect was 48.2%. In these patients, a negative JCV PCR is highly suggestive of other neurological conditions.

摘要

目的

评估巴西艾滋病患者脑脊液样本中是否存在JC病毒DNA，这些患者有中枢神经系统白质局灶性病变且无占位效应，符合进行性多灶性白质脑病（PML）。

方法

对有神经症状且CT扫描显示中枢神经系统白质局灶性病变且无占位效应提示PML的艾滋病患者的脑脊液样本，以及患有非PML神经疾病的艾滋病和非艾滋病患者的脑脊液样本进行PCR检测JC病毒DNA。用于扩增JC病毒T抗原区域的引物产生一个173bp的片段。使用BamH1消化PCR产物来确认JC病毒的存在。

结果

120份非PML脑脊液样本中119份的JC病毒DNA PCR检测为阴性（特异性=99.2%）。56份来自有中枢神经系统白质局灶性病变且无占位效应的艾滋病患者的脑脊液样本中，48.2%（27/56）的JC病毒DNA呈阳性。在23/29（79.3%）JC病毒DNA阴性病例中，发现了脑炎性病变的其他病因。没有JC病毒DNA阳性病例显示有其他诊断结果。