Cao Wenling, Wang Aijun, Jing Duohui, Gong Yandao, Zhao Nanming, Zhang Xiufang
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
J Biomater Sci Polym Ed. 2005;16(11):1379-94. doi: 10.1163/156856205774472308.
In order to develop a novel biomaterial, films of chitosan blended with poly(3-hydroxybutyrate) (PHB) were prepared by an emulsion blending technique and their properties were characterized. Scanning electron microscopy (SEM) showed that PHB microspheres were formed and were entrapped in chitosan matrices, which made the film surface rough. With increasing PHB content, the roughness of the film surface increased, while the swelling capability of the films decreased. In a wet state, the blended films exhibited a lower elastic modulus, a higher elongation-at-break and a higher tensile strength compared with chitosan films. Cell-culture experiments revealed that the blended films had better cytocompatibility than chitosan films. To explore the potential application of the blended material in tissue engineering, the porous blended scaffolds were fabricated and their pore morphology was observed by SEM. The results revealed that not only pore structure but also pore wall morphology of the blended scaffolds could be controlled by selecting the parameters of the fabrication process. These advantageous properties indicate that the blended chitosan/PHB material is promising for tissue engineering applications.
为了开发一种新型生物材料，采用乳液共混技术制备了壳聚糖与聚（3-羟基丁酸酯）（PHB）共混的薄膜，并对其性能进行了表征。扫描电子显微镜（SEM）显示形成了PHB微球并被包裹在壳聚糖基质中，这使得薄膜表面粗糙。随着PHB含量的增加，薄膜表面粗糙度增加，而薄膜的溶胀能力下降。在湿态下，与壳聚糖薄膜相比，共混薄膜表现出较低的弹性模量、较高的断裂伸长率和较高的拉伸强度。细胞培养实验表明，共混薄膜比壳聚糖薄膜具有更好的细胞相容性。为了探索这种共混材料在组织工程中的潜在应用，制备了多孔共混支架，并通过SEM观察其孔形态。结果表明，通过选择制备工艺参数，不仅可以控制共混支架的孔结构，还可以控制孔壁形态。这些有利性能表明，壳聚糖/PHB共混材料在组织工程应用中具有广阔前景。
