用于组织工程应用的聚（3-羟基丁酸酯）/壳聚糖共混支架的制备与表征
Preparation and characterization of poly (hydroxy butyrate)/chitosan blend scaffolds for tissue engineering applications.
作者信息
Karbasi Saeed, Khorasani Saied Nouri, Ebrahimi Somayeh, Khalili Shahla, Fekrat Farnoosh, Sadeghi Davoud
机构信息
Department of Biomaterials and Tissue Engineering, School of Advance Technology in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran.
出版信息
Adv Biomed Res. 2016 Nov 28;5:177. doi: 10.4103/2277-9175.188490. eCollection 2016.
BACKGROUND
Poly (hydroxy butyrate) (PHB) is a biodegradable and biocompatible polymer with good mechanical properties. This polymer could be a promising material for scaffolds if some features improve.
MATERIALS AND METHODS
In the present work, new PHB/chitosan blend scaffolds were prepared as a three-dimensional substrate in cartilage tissue engineering. Chitosan in different weight percent was added to PHB and solved in trifluoroacetic acid. Statistical Taguchi method was employed in the design of experiments.
RESULTS
The Fourier-transform infrared spectroscopy test revealed that the crystallization of PHB in these blends is suppressed with increasing the amount of chitosan. Scanning electron microscopy images showed a thin and rough top layer with a nodular structure, supported with a porous sub-layer in the surface of the scaffolds. degradation rate of the scaffolds was higher than pure PHB scaffolds. Maximum degradation rate has been seen for the scaffold with 90% wt. NaCl and 40% wt. chitosan.
CONCLUSIONS
The obtained results suggest that these newly developed PHB/chitosan blend scaffolds may serve as a three-dimensional substrate in cartilage tissue engineering.
背景
聚羟基丁酸酯（PHB）是一种具有良好机械性能的可生物降解且生物相容的聚合物。如果某些特性得到改善，这种聚合物可能成为用于支架的有前景的材料。
材料与方法
在本研究中，制备了新型PHB/壳聚糖共混支架，作为软骨组织工程中的三维基质。将不同重量百分比的壳聚糖添加到PHB中，并溶解于三氟乙酸中。实验设计采用统计田口方法。
结果
傅里叶变换红外光谱测试表明，随着壳聚糖含量的增加，这些共混物中PHB的结晶受到抑制。扫描电子显微镜图像显示，支架表面有一层薄而粗糙的顶层，具有结节状结构，下层为多孔结构。支架的降解速率高于纯PHB支架。含90%重量的氯化钠和40%重量的壳聚糖的支架降解速率最高。
结论
所得结果表明，这些新开发的PHB/壳聚糖共混支架可作为软骨组织工程中的三维基质。
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