DenBesten P K, Heffernan L M, Featherstone J D, Shields C P
Forsyth Dental Center, Boston, MA 02115.
Arch Oral Biol. 1992;37(6):459-62. doi: 10.1016/0003-9969(92)90100-m.
Chronic fluoride exposure in vivo results in alterations in the formation of mineralizing tissues. One possible mechanism for the formation of fluorosed tooth enamel and bone is a binding of fluoride to matrix proteins, resulting in an alteration in their structure and function. Studies were designed to investigate fluoride binding to matrix proteins in vivo and their possible role in fluorosis. Rats were given either 0 or 100 parts/10(6) fluoride in drinking water for 6 weeks to allow the formation of fluorotic mineralizing tissues. The animals were killed by CO2 inhalation, and the enamel and bone were analysed for fluoride and calcium. Matrix binding by fluoride in enamel was determined after extraction of proteins from undemineralized matrix. In bone, the matrix was demineralized and F, Ca and P were determined in both ashed and unashed samples. The studies showed ionic binding of fluoride to the matrix in both enamel and bone, possibly associated with calcium binding by the matrix. There was no difference in the amount of matrix-bound fluoride in control as compared to fluorosed bone or maturation-stage enamel. This indicates that although matrix proteins can bind fluoride, it is not likely that this mechanism is important in the formation of fluorosed mineralizing tissues.
体内长期接触氟会导致矿化组织形成的改变。氟斑牙釉质和骨骼形成的一种可能机制是氟与基质蛋白结合，导致其结构和功能发生改变。本研究旨在探讨体内氟与基质蛋白的结合情况及其在氟中毒中的可能作用。给大鼠饮用含0或100 ppm氟的水6周，以形成氟中毒的矿化组织。通过吸入二氧化碳处死动物，并分析牙釉质和骨骼中的氟和钙含量。从未脱矿基质中提取蛋白质后，测定氟在牙釉质中与基质的结合情况。在骨骼中，将基质脱矿，并测定灰化和未灰化样品中的氟、钙和磷含量。研究表明，氟在牙釉质和骨骼中均与基质发生离子结合，可能与基质结合钙有关。与氟斑骨或成熟阶段牙釉质相比，对照中基质结合氟的量没有差异。这表明，虽然基质蛋白可以结合氟，但这种机制在氟斑矿化组织的形成中可能并不重要。
