茴芹果实（茴芹属，伞形科）的流浸膏和精油的抗真菌活性

Antifungal activity of fluid extract and essential oil from anise fruits (Pimpinella anisum L., Apiaceae).

作者信息

Kosalec Ivan, Pepeljnjak Stjepan, Kustrak Danica

机构信息

Department of Microbiology Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.

出版信息

Acta Pharm. 2005 Dec;55(4):377-85.

PMID:16375827

Abstract

Antifungal activities of fluid extract and essential oil obtained from anise fruits Pimpinella anisum L. (Apiaceae) were tested in vitro on clinical isolates of seven species of yeasts and four species of dermatophytes. Diffusion method with cylinders and the broth dilution method were used for antifungal activity testing. Anise fluid extract showed antimycotic activity against Candida albicans, C. parapsilosis, C. tropicalis, C. pseudotropicalis and C. krusei with MIC values between 17 and 20% (v/v). No activity was noticed against C. glabrata, and anis fruits extracts showed growth promotion activity on Geotrichum spp. Anise fruits extract inhibited the growth of dermatophyte species (Trichophyton rubrum, T. mentagrophytes, Microsporum canis and M. gypseum) with MIC values between 1.5 and 9.0% (V/V). Anise essential oil showed strong antifungal activity against yeasts with MIC lower than 1.56% (V/V) and dermatophytes with MIC lower than 0.78% (V/V). Significant differences in antifungal activities were found between anise fluid extract and anise essential oil (p<0.01). Anise essential oil exhibited stronger antifungal activities against yeasts and dermatophytes with MIC values between 0.10 and 1.56% (V/V), respectively.

摘要

对茴芹果实（茴芹属，伞形科）提取的流浸膏和精油的抗真菌活性进行了体外测试，测试对象为七种酵母临床分离株和四种皮肤癣菌。采用滤纸片扩散法和肉汤稀释法进行抗真菌活性测试。茴芹流浸膏对白色念珠菌、近平滑念珠菌、热带念珠菌、伪热带念珠菌和克柔念珠菌表现出抗真菌活性，其最低抑菌浓度（MIC）值在17%至20%（v/v）之间。对光滑念珠菌未观察到活性，且茴芹果实提取物对丝孢酵母属表现出促生长活性。茴芹果实提取物抑制皮肤癣菌（红色毛癣菌、须癣毛癣菌、犬小孢子菌和石膏样小孢子菌）的生长，其MIC值在1.5%至9.0%（V/V）之间。茴芹精油对酵母表现出较强的抗真菌活性，MIC低于1.56%（V/V），对皮肤癣菌的MIC低于0.78%（V/V）。发现茴芹流浸膏和茴芹精油的抗真菌活性存在显著差异（p<0.01）。茴芹精油对酵母和皮肤癣菌分别表现出更强的抗真菌活性，MIC值分别在0.10%至1.56%（V/V）之间。