Jørgensen Lars M, Hauser Frank, Cazzamali Giuseppe, Williamson Michael, Grimmelikhuijzen Cornelis J P
Center for Functional and Comparative Insect Genomics, Department of Cell Biology and Comparative Zoology, Institute of Biology, University of Copenhagen, DK-2100 Copenhagen, Denmark.
Biochem Biophys Res Commun. 2006 Feb 10;340(2):696-701. doi: 10.1016/j.bbrc.2005.12.062. Epub 2005 Dec 20.
SIFamide is the short name and also the C terminus of the Drosophila neuropeptide AYRKPPFNGSIFamide. SIFamide has been isolated or predicted from various insects and crustaceans, and appears to be extremely well conserved among these arthropods. However, the function of this neuropeptide is still enigmatic. Here, we have identified the Drosophila gene (CG10823) coding for the SIFamide receptor. When expressed in Chinese hamster ovary cells, the receptor is only activated by Drosophila SIFamide (EC(50), 2x10(-8)M) and not by a library of 32 other insect neuropeptides and eight biogenic amines. Database searches revealed SIFamide receptor orthologues in the genomes from the malaria mosquito Anopheles gambiae, the silkworm Bombyx mori, the red flour beetle Tribolium castaneum, and the honey bee Apis mellifera. An alignment of the five insect SIFamide or SIFamide-like receptors showed, again, an impressive sequence conservation (67-77% amino acid sequence identities between the seven-transmembrane areas; 82-87% sequence similarities). The identification of well-conserved SIFamide receptor orthologues in all other insects with a sequenced genome, suggests that the SIFamide/receptor couple must have an essential function in arthropods. This paper is the first report on the identification of a SIFamide receptor.
SIF酰胺是果蝇神经肽AYRKPPFNGSIF酰胺的简称，也是其C末端。SIF酰胺已从多种昆虫和甲壳类动物中分离出来或被预测存在，并且在这些节肢动物中似乎高度保守。然而，这种神经肽的功能仍然未知。在此，我们鉴定出了编码SIF酰胺受体的果蝇基因（CG10823）。当在中国仓鼠卵巢细胞中表达时，该受体仅被果蝇SIF酰胺激活（半数有效浓度，2×10⁻⁸M），而不被其他32种昆虫神经肽和8种生物胺的文库激活。数据库搜索揭示了在冈比亚疟蚊、家蚕、赤拟谷盗和蜜蜂的基因组中存在SIF酰胺受体直系同源物。对这五种昆虫的SIF酰胺或SIF酰胺样受体进行比对，再次显示出令人印象深刻的序列保守性（七个跨膜区域之间的氨基酸序列同一性为67 - 77%；序列相似性为82 - 87%）。在所有其他已测序基因组的昆虫中都鉴定出了高度保守的SIF酰胺受体直系同源物，这表明SIF酰胺/受体对在节肢动物中必定具有重要功能。本文是关于鉴定SIF酰胺受体的首次报道。
