Belmont Martin, Cazzamali Giuseppe, Williamson Michael, Hauser Frank, Grimmelikhuijzen Cornelis J P
Center for Functional and Comparative Insect Genomics, Department of Cell Biology and Comparative Zoology, Institute of Biology, University of Copenhagen, DK-2100 Copenhagen, Denmark.
Biochem Biophys Res Commun. 2006 May 26;344(1):160-5. doi: 10.1016/j.bbrc.2006.03.117. Epub 2006 Apr 17.
The mosquito Anopheles gambiae is an important vector for malaria, which is one of the most serious human parasitic diseases in the world, causing up to 2.7 million deaths yearly. To contribute to our understanding of A. gambiae and to the transmission of malaria, we have now cloned four evolutionarily related G protein-coupled receptors (GPCRs) from this mosquito and expressed them in Chinese hamster ovary cells. After screening of a library of thirty-three insect or other invertebrate neuropeptides and eight biogenic amines, we could identify (de-orphanize) three of these GPCRs as: an adipokinetic hormone (AKH) receptor (EC(50) for A. gambiae AKH, 3x10(-9)M), a corazonin receptor (EC(50) for A. gambiae corazonin, 4x10(-9)M), and a crustacean cardioactive peptide (CCAP) receptor (EC(50) for A. gambiae CCAP, 1x10(-9)M). The fourth GPCR remained an orphan, although its close evolutionary relationship to the A. gambiae and other insect AKH receptors suggested that it is a receptor for an AKH-like peptide. This is the first published report on evolutionarily related AKH, corazonin, and CCAP receptors in mosquitoes.
冈比亚按蚊是疟疾的重要传播媒介，疟疾是世界上最严重的人类寄生虫病之一，每年造成多达270万人死亡。为了增进我们对冈比亚按蚊以及疟疾传播的了解，我们现已从这种蚊子中克隆出四种进化相关的G蛋白偶联受体（GPCR），并在中国仓鼠卵巢细胞中进行了表达。在筛选了一个包含33种昆虫或其他无脊椎动物神经肽以及8种生物胺的文库后，我们可以将其中三种GPCR鉴定（去孤儿化）为：一种脂肪动激素（AKH）受体（冈比亚按蚊AKH的EC(50)为3×10(-9)M）、一种心钠素受体（冈比亚按蚊心钠素的EC(50)为4×10(-9)M）和一种甲壳类心脏活性肽（CCAP）受体（冈比亚按蚊CCAP的EC(50)为1×10(-9)M）。尽管第四种GPCR与冈比亚按蚊及其他昆虫的AKH受体有密切的进化关系，提示它是一种类AKH肽的受体，但它仍是孤儿受体。这是关于蚊子中进化相关的AKH、心钠素和CCAP受体的首篇发表报告。
