Amaral Joana S, Casal Susana, Torres Duarte, Seabra Rosa M, Oliveira Beatriz P P
REQUIMTE, Serviço de Farmacognosia, Faculdade de Farmácia, Rua Anibal Cunha, 164, 4099-030 Porto, Portugal.
Anal Sci. 2005 Dec;21(12):1545-8. doi: 10.2116/analsci.21.1545.
A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed; the best results were obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E homologues.
报道了一种用于测定榛子中生育酚和生育三烯酚的正相高效液相色谱（NP-HPLC）方法。测定了三种提取程序（有皂化和无皂化）；采用简单的固液萃取程序获得了最佳结果。使用Inertsil 5 SI柱，以己烷/1,4-二氧六环（95.5:4.5，v/v）等度洗脱，流速为0.7 mL/min，实现了色谱分离。流出物通过二极管阵列串联荧光检测器进行监测。所有化合物在短时间内（17分钟）分离。该方法被证明是快速、灵敏、可重现且准确的，能够同时测定所有维生素E同系物。
